Differential properties of human stromal cells from bone marrow, adipose, liver and cardiac tissues
Differential properties of human stromal cells from bone marrow, adipose, liver and cardiac tissues
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DOI：
10.1016/j.jcyt.2015.07.009
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发表时间：
2015-11-01
期刊：
影响因子：
4.5
通讯作者：
McNiece, Ian
中科院分区：
文献类型：
--
作者：
Kellner, Joshua;Sivajothi, Santhosh;McNiece, Ian
背景目标。间充质基质细胞（MSC）源自骨髓和脂肪组织等多种组织，目前正在针对一系列疾病的临床试验中进行评估。事实上，身体的所有组织都含有基质细胞，但尚不清楚这些来源在表型和功能上是否相似。方法。我们从多种人体组织中分离了基质细胞，包括骨髓（BM-MSC）、心脏（心脏基质，HS）、脂肪（脂肪基质，AS）和肝脏（肝基质，LS），并比较了这些基质细胞群的形态、表型和功能特性。结果。每个群体的细胞表型是相同的，即CD105+、CD73+、CD90+、CD34-和CD45-。此外，形态和分化潜力具有可比性。共培养研究揭示了 BM-MSC、AS 和 LS 与造血细胞或肿瘤细胞具有相似的支持潜力。相比之下，HS 对两种细胞类型的增殖均产生显着抑制，同时肿瘤细胞的活力显着丧失，这表明 HS 在肿瘤细胞增殖方面具有独特的功能特性。结论。尽管来自不同组织的基质细胞具有相似的形态和表型，但它们的功能特性各不相同，因此在用于非同源环境之前需要对基质细胞进行严格评估。 HS可能在抑制心脏肿瘤细胞增殖方面发挥关键作用，这为肿瘤发生率低提供了原因。鉴于 BM-MSC 和 AS 的肿瘤支持特性，在心脏组织中使用这些细胞可能会导致用肿瘤支持微环境取代肿瘤抑制基质。
Background aims. Mesenchymal stromal cells (MSCs), derived from several tissues including bone marrow and adipose tissue, are being evaluated in clinical trials for a range of diseases. Virtually all tissues of the body contain stromal cells, yet it is unknown whether these sources are similar in phenotype and function. Methods. We have isolated stromal cells from several human tissues including bone marrow (BM-MSCs), heart (heart stroma, HS), adipose (adipose stroma, AS) and liver (liver stroma, LS) and compared the morphology, phenotype and functional properties of these stromal cell populations. Results. The cellular phenotype of each population was identical, namely, CD105+, CD73+, CD90+, CD34- and CD45-. In addition, morphology and differentiation potential were comparable. Co-culture studies revealed similar supportive potential of BM-MSCs, AS and LS with hematopoietic cells or tumor cells. In contrast, significant inhibition of proliferation of both cells types was obtained with HS, with significant loss of viability with tumor cells, demonstrating a unique functional property of HS with regard to tumor cell proliferation. Conclusions. Although stromal cells from different tissues have similar morphology and phenotype, their functional properties vary, requiring critical evaluation of stromal cells before use in non-homologous settings. HS may play a key role in inhibiting proliferation of tumor cells in the heart, providing the reason for the low occurrence of tumor development. Given the tumor-supportive property of BM-MSCs and AS, the use of these cells in cardiac tissue may result in replacement of a tumor-inhibitory stroma with a tumor-supportive microenvironment.