Molecular, forensic and haplotypic inconsistencies regarding the identity of the Ekaterinburg remains
Molecular, forensic and haplotypic inconsistencies regarding the identity of the Ekaterinburg remains
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DOI：
10.1080/03014460310001652257
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发表时间：
2004-03-01
影响因子：
1.7
通讯作者：
Mountain, JL
中科院分区：
文献类型：
--
作者：
Knight, A;Zhivotovsky, LA;Mountain, JL
背景资料：在俄罗斯叶卡捷琳堡附近出土的一组人类遗骸被认为是俄罗斯罗曼诺夫皇室及其医生和仆人的遗骸。这一结论在DNA测试结果公布后被俄罗斯政府正式接受。发表的研究报告没有讨论重大的法医差异，关于埋葬地点和遗骸的信息包括违规行为。此外，它关于罗曼诺夫身份的结论是基于表明污染的分子行为，而不是内源性DNA。已发表的声明称，通过PCR扩增了9个个体的单一片段中降解DNA的1223 bp区域，然后获得了来自该片段的PCR产物序列，而没有克隆，这表明叶卡捷琳堡样本被未降解的高分子量"新鲜“DNA污染。注意到严重违反标准法医实践，事实不一致，和分子行为，使声称的身份无效，我们试图复制原始DNA研究的结果。主题：我们分析了提取的mtDNA样本的遗物的大公夫人伊丽莎白，皇后Alexandra.Results的妹妹：在多个PCR靶和产物的克隆，我们没有观察到完整的mtDNA单倍型匹配，亚历山德拉报道。伊丽莎白的共识单倍型不同的亚历山德拉在four sites.Conclusion：考虑到分子和法医学的不一致，身份的叶卡捷琳堡仍然没有建立。伊丽莎白的mtDNA单倍型结果为叶卡捷琳堡遗骸的身份提供了另一条相互矛盾的证据。
Background: A set of human remains unearthed near Ekaterinburg, Russia has been attributed to the Romanov Imperial Family of Russia and their physician and servants. That conclusion was officially accepted by the Russian government following publication of DNA tests that were widely publicized. The published study included no discussion of major forensic discrepancies and the information regarding the burial site and remains included irregularities. Furthermore, its conclusion of Romanov identity was based on molecular behaviour that indicates contamination rather than endogenous DNA. The published claim to have amplified by PCR a 1223 bp region of degraded DNA in a single segment for nine individuals and then to have obtained sequence of PCR products derived from that segment without cloning indicates that the Ekaterinburg samples were contaminated with non-degraded, high molecular weight, 'fresh' DNA.Aim: Noting major violations of standard forensic practices, factual inconsistencies, and molecular behaviours that invalidate the claimed identity, we attempted to replicate the findings of the original DNA study.Subject: We analysed mtDNA extracted from a sample of the relic of Grand Duchess Elisabeth, sister of Empress Alexandra.Results: Among clones of multiple PCR targets and products, we observed no complete mtDNA haplotype matching that reported for Alexandra. The consensus haplotype of Elisabeth differs from that reported for Alexandra at four sites.Conclusion: Considering molecular and forensic inconsistencies, the identity of the Ekaterinburg remains has not been established. Our mtDNA haplotype results for Elisabeth provide yet another line of conflicting evidence regarding the identity of the Ekaterinburg remains.