Sensitivity of bacteria to diamond nanoparticles of various size differs in gram-positive and gram-negative cells
Sensitivity of bacteria to diamond nanoparticles of various size differs in gram-positive and gram-negative cells
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DOI：
10.1111/1574-6968.12373
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发表时间：
2014-02-01
影响因子：
2.1
通讯作者：
Kromka, Alexander
中科院分区：
文献类型：
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作者：
Beranova, Jana;Seydlova, Gabriela;Kromka, Alexander
本研究考察了金刚石纳米颗粒（DNPs）的大小和表面终止对其对大肠杆菌和枯草芽孢杆菌的抗菌活性的影响。采用动态光散射和x射线衍射技术测定了DNPs的平均尺寸和分布。通过x射线光电子能谱对DNPs的化学成分进行了研究，结果表明：DNPs > ~ 5nm氧化颗粒具有较高的氧含量。采用活菌计数法评价DNPs的抑菌潜力。一般来说,E。大肠杆菌对DNPs的敏感性高于b。细小。然而，在所有检测的DNPs存在的情况下，b。枯草菌落的大小和形态发生了变化。抑菌活性不仅受DNP浓度的影响，还受DNP大小和形态的影响。而未经处理的5nm DNPs则对ste最有效。18 ~ 50 nm DNPs对大肠杆菌的抑菌活性较高。细小。透射电镜显示DNPs与细菌表面相互作用，可能影响重要的细胞功能。我们认为DNPs干扰细菌细胞壁和/或膜的通透性，并阻碍b。subtiliscolony蔓延。
In this study, the influence of the size and surface termination of diamond nanoparticles (DNPs) on their antibacterial activity againstEscherichia coliandBacillus subtiliswas assessed. The average size and distribution of DNPs were determined by dynamic light scattering and X-ray diffraction techniques. The chemical composition of the DNPs studied by X-ray photoelectron spectroscopy showed that DNPs > 5 nm and oxidized particles have a higher oxygen content. The antibacterial potential of DNPs was assessed by the viable count method. In general,E. coliexhibited a higher sensitivity to DNPs thanB. subtilis. However, in the presence of all the DNPs tested, theB. subtiliscolonies exhibited altered size and morphology. Antibacterial activity was influenced not only by DNP concentration but also by DNP size and form. Whereas untreated 5-nm DNPs were the most effective againstE. coli, the antibacterial activity of 18–50-nm DNPs was higher againstB. subtilis. Transmission electron microscopy showed that DNPs interact with the bacterial surface, probably affecting vital cell functions. We propose that DNPs interfere with the permeability of the bacterial cell wall and/or membrane and hinderB. subtiliscolony spreading.