The effect of stress-inducible extracellular Hsp72 on human neutrophil chemotaxis: A role during acute intense exercise
The effect of stress-inducible extracellular Hsp72 on human neutrophil chemotaxis: A role during acute intense exercise
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DOI：
10.1080/10253890802309853
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发表时间：
2009-01
期刊：
影响因子：
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通讯作者：
E. Ortega;M. D. Hinchado;L. Martín-Cordero;A. Asea
中科院分区：
文献类型：
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作者：
E. Ortega;M. D. Hinchado;L. Martín-Cordero;A. Asea
我们研究了72 kDa细胞外热休克蛋白（Hsp 72，一种应激诱导蛋白）在久坐女性进行单次剧烈运动期间调节中性粒细胞趋化性的生理作用，以及可能参与调节的各种细胞机制。对于每个志愿者，我们评估中性粒细胞趋化性和血清Hsp 72浓度之前和之后立即进行一轮运动（1小时的自行车功率计在70%VO2最大值），24小时后。这两个参数被认为是刺激的运动，并已恢复到基础值24小时后。在体外，当中性粒细胞与生理浓度的Hsp 72和100倍以上的浓度孵育时，趋化性呈剂量依赖性增加。的趋化性是更大的中性粒细胞与运动后的热休克蛋白72浓度比与基础浓度孵育，这表明这种蛋白质的刺激中性粒细胞趋化性的背景下，由激烈的运动的生理作用。100 × Hsp 72浓度刺激趋化性甚至更强。此外，发现Hsp 72在生理浓度下对中性粒细胞具有化学引诱剂和趋化动力学作用，运动后的这些作用显着大于基础Hsp 72浓度。当Toll样受体2（TLR-2）被阻断时，Hsp 72诱导的中性粒细胞趋化性的刺激消失，磷脂酰肌醇-3-激酶（PI 3 K）、细胞外信号调节激酶（ERK）和核转录因子κ B（NF-κB）也参与了这一信号传导过程。没有观察到变化，但是，中性粒细胞内钙离子水平响应热休克蛋白72。总之，生理浓度的应激蛋白Hsp 72通过TLR-2及其辅因子CD 14刺激人中性粒细胞趋化性，涉及ERK、NF-κB和PI 3 K，但不涉及iCa 2+作为细胞内信使。此外，热休克蛋白72似乎参与刺激的趋化性诱导的一轮激烈的运动进行久坐的妇女。
We studied the physiological role of the 72 kDa extracellular heat shock protein (Hsp72, a stress-inducible protein) in modulating neutrophil chemotaxis during a single bout of intense exercise performed by sedentary women, together with various cell mechanisms potentially involved in the modulation. For each volunteer, we evaluated neutrophil chemotaxis and serum Hsp72 concentration before and immediately after a single bout of exercise (1 h on a cycle ergometer at 70% VO2 max), and 24 h later. Both parameters were found to be stimulated by the exercise, and had returned to basal values 24 h later. In vitro, there was a dose-dependent increase in chemotaxis when neutrophils were incubated both with physiological Hsp72 concentrations and with a 100 × greater concentration. The chemotaxis was greater when the neutrophils were incubated with the post-exercise Hsp72 concentration than with the basal concentration, suggesting a physiological role for this protein in the context of the stimulation of neutrophil chemotaxis by intense exercise. The 100 × Hsp72 concentration stimulated chemotaxis even more strongly. In addition, Hsp72 was found to have chemoattractant and chemokinetic effects on the neutrophils at physiological concentrations, with these effects being significantly greater with the post-exercise than with the basal Hsp72 concentration. The Hsp72-induced stimulation of neutrophil chemotaxis disappeared when the toll-like receptor 2 (TLR-2) was blocked, and phosphatidylinositol-3-kinase (PI3K), extracellular signal-regulated kinase (ERK), and nuclear transcription factor kappa B (NF-κB) were also found to be involved in the signaling process. No changes were observed, however, in neutrophil intracellular calcium levels in response to Hsp72. In conclusion, physiological concentrations of the stress protein Hsp72 stimulate human neutrophil chemotaxis through TLR-2 with its cofactor CD14, involving ERK, NF-κB, and PI3K, but not iCa2 + , as intracellular messengers. In addition, Hsp72 seems to participate in the stimulation of chemotaxis induced by a single bout of intense exercise performed by sedentary women.