Water-borne Campylobacter jejuni infection in a Danish town---a 6-week continuous source outbreak.
Water-borne Campylobacter jejuni infection in a Danish town---a 6-week continuous source outbreak.
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DOI：
10.1111/j.1469-0691.1998.tb00348.x
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发表时间：
1998-01-01
期刊：
影响因子：
--
通讯作者：
Molbak, Kare
中科院分区：
文献类型：
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作者：
Engberg, Jorgen;Gerner-Smidt, Peter;Molbak, Kare
目的：确定1995- 1996年丹麦一起空肠弯曲菌暴发的病因和疾病特征。方法：对培养确诊病例和无细菌学诊断的居民进行回顾性随访研究。通过限制性内切酶分析（REA）、脉冲场凝胶电泳（PFGE）和核糖体分型对储存的临床和环境分离株进行血清分型和基因分型。结果：从110名居民和游客中分离出空肠弯曲菌。然而，根据电话调查估计，约有2400人受到疫情影响。从社区自来水厂获得的水样中含有空肠弯曲菌血清型O2，除一株外，所有30株储存的暴发分离株的血清型均相同。水和临床分离株也显示出相同的DNA谱，除了单一菌株显示出不同的血清型。供水受到污染的原因可追溯到污水管破裂造成的地下水污染。结论：对该镇的培养确诊和非培养确诊病例进行回顾性和人口流行病学调查，并结合分离株的分型，对确定暴发的程度和原因至关重要。
OBJECTIVE: To determine the cause and characteristics of illness of a Campylobacter jejuni outbreak in Denmark in 1995--96. METHODS: A retrospective follow-up study was designed for culture-confirmed cases and for residents without a bacteriologic diagnosis. Stored clinical and environmental isolates were analyzed by serotyping and genotyping with restriction endonuclease analysis (REA), pulsed-field gel electrophoresis (PFGE) and ribotyping. RESULTS: Campylobacter jejuni was isolated from 110 residents and visitors to the area. However, an estimate based on a telephone survey indicated that some 2400 people were affected by the outbreak. Water samples obtained from the community waterworks contained Campylobacter jejuni serotype O2, the same serotype as in all but one of the 30 stored isolates from the outbreak. The water and clinical isolates also showed the same DNA profile, except for the single strain showing the distinct serotype. The contamination of the water supply was traced back to contamination of ground water due to a break in a sewage pipe. CONCLUSIONS: A retrospective and demographic epidemiologic investigation of both culture-confirmed and non-culture-confirmed cases in the town combined with typing of the isolates was crucial in defining the extent and cause of the outbreak.