The role of the microenvironment in human immunodeficiency virus-associated classical Hodgkin lymphoma
The role of the microenvironment in human immunodeficiency virus-associated classical Hodgkin lymphoma
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DOI：
10.1111/his.12458
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发表时间：
2014-12-01
期刊：
影响因子：
6.4
通讯作者：
Naresh, Kikkeri N.
中科院分区：
文献类型：
--
作者：
Koulis, Athanasios;Trivedi, Pritesh;Naresh, Kikkeri N.
目的探讨人类免疫缺陷病毒（PLWH）感染者经典型霍奇金淋巴瘤（cHL）的微环境特征。其目的是确定，然后量化的免疫细胞存在于microenvironment.Methods和resultsTen样本的cHL PLWH进行了比较，10个样本的cHL从一般人群中使用组织芯片技术和免疫组化。切片用CD30、CD3、CD4、CD8、CD20、CD68 R、CD56、CD57、CD123、FoxP3和颗粒酶B的抗体免疫染色。在PLWH中诊断的cHL中观察到CD4（+）T细胞、CD56（+）细胞、CD57（+）细胞、CD123（+）细胞和B细胞的统计学显著减少以及FoxP3（+）CD8（+）细胞数量的增加。CD8（+）T细胞、CD4（+）FoxP3（+）T细胞和巨噬细胞的数量无显著差异。
AimsTo characterize the microenvironment of classical Hodgkin lymphoma (cHL) in people living with human immunodeficiency virus (PLWH). The objective was to identify and then quantify the immune cells present in the microenvironment.Methods and resultsTen samples of cHL from PLWH were compared with 10 samples of cHL from the general population using tissue microarray technology and immunohistochemistry. Sections were immunostained with antibodies for CD30, CD3, CD4, CD8, CD20, CD68R, CD56, CD57, CD123, FoxP3 and granzyme B. A statistically significant reduction of CD4(+) T cells, CD56(+) cells, CD57(+) cells, CD123(+) cells and B cells and an increase in numbers in FoxP3(+)CD8(+) cells was observed in cHL diagnosed in PLWH. No significant differences were seen in the number of CD8(+) T cells, CD4(+)FoxP3(+) T cells and macrophages.ConclusionThere are considerable differences in the microenvironment of cHL occurring with and without HIV.