A fluorescent biosensor based on carbon dots-labeled oligodeoxyribonucleotide and graphene oxide for mercury (II) detection
A fluorescent biosensor based on carbon dots-labeled oligodeoxyribonucleotide and graphene oxide for mercury (II) detection
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DOI：
10.1016/j.bios.2014.07.085
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发表时间：
2015-01-15
影响因子：
12.6
通讯作者：
Yang, Mei
中科院分区：
文献类型：
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作者：
Cui, Xin;Zhu, Lei;Yang, Mei
作为碳材料家族的最新两个成员，碳点（CD）和氧化石墨烯（GO）具有许多优异的光学性能，具有广泛的应用前景。在这项工作中，我们成功合成了高量子产率的CD，并将其标记在寡脱氧核糖核苷酸（ODN）上。所得 CD 标记的寡脱氧核糖核苷酸 (ODN-CD) 的荧光通过荧光共振能量转移被 GO 猝灭。在 Hg2+ 存在的情况下，由于 T-Hg2+-T 双链体的形成，ODN-CD 从 GO 中释放出来，从而恢复了荧光。根据这一理论，我们设计了一种基于CDs标记的寡脱氧核糖核苷酸和GO的简单、高灵敏度和选择性荧光Hg2+传感器，无需复杂、昂贵且耗时的操作。在最佳条件下，相对荧光强度与Hg2+浓度在5-200 nM范围内呈线性关系（R-2=0.974）。目前基于 GO 的传感器系统对多种金属离子中的 Hg2+ 具有高度选择性，检测限为 2.6 nM。该方法可靠，已成功应用于实际样品中Hg2+的检测。 (C) 2014 Elsevier B.V. 保留所有权利。
As the newest two members of the carbon materials family, carbon dots (CDs) and graphene oxide (GO) possess many excellent optical properties resulting in a wide range of applications. In this work, we successfully synthesized CDs with a high-quantum-yield, and labeled them on oligodeoxyribonucleotide (ODN). The fluorescence of resultant CDs-labeled oligodeoxyribonucleotide (ODN-CDs) was quenched by GO via fluorescence resonance energy transfer. In the presence of Hg2+, the fluorescence was recovered by the release of ODN-CDs from GO due to the formation of T-Hg2+-T duplex. In the light of this theory, we designed a simple, highly sensitive and selective fluorometric Hg2+ sensor based on CDs-labeled oligodeoxyribonucleotide and GO without complicated, costly and time-consuming operations. Under the optimal conditions, a linear relationship was obtained between relative fluorescence intensity and the concentration of Hg2+ in the range of 5-200 nM (R-2=0.974). The present GO-based sensor system is highly selective toward Hg2+ over a wide range of metal ions and has a detection limit of 2.6 nM. This method is reliable, and has been successfully applied for the detection of Hg2+ in practical samples. (C) 2014 Elsevier B.V. All rights reserved.