Mapping of 228 ESTs and 26 genes into an integrated physical and genetic map of human chromosome 17.
Mapping of 228 ESTs and 26 genes into an integrated physical and genetic map of human chromosome 17.
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将 228 个 EST 和 26 个基因映射到人类 17 号染色体的综合物理和遗传图谱中。
DOI：
10.1006/geno.1997.4906
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发表时间：
1997
期刊：
影响因子：
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通讯作者：
Casey,G
中科院分区：
文献类型：
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作者：
Plummer,SJ;Simmons,JA;Adams,L;Casey,G
我们通过使用一组17号染色体缺失的体细胞杂交，将17号染色体的遗传和物理图谱数据整合到26个储存箱中。104个短串联重复序列和STS标记被定位到这些库中，使288个EST和26个基因的排序成为可能，其中142个EST以前没有被定位在17号染色体上。利用这个杂交小组，已经整合了几个遗传图谱的作图信息，以及通过辐射杂交和STS含量作图获得的信息。虽然现有的17号染色体的作图信息与之前定位的许多EST大致一致，但这里提供的图谱进一步细化了EST的位置，并展示了在17q24-q25区域发现的一些差异。我们将这些差异归因于这样一个事实，即当前的辐射杂交面板被选择用于保留17q25的胸苷激酶基因，以及该区域低浓度的YAC重叠群。这些数据说明了组合多种测绘技术以获得最大精度的好处。不同方法开发的图谱的整合将产生最准确的基因组图谱，然后可以用于产生用于染色体测序的大插入克隆重叠群。此外，由EST生成的准确的转录图谱将大大加快与疾病基因连锁的基因的分离。
We have integrated genetic and physical mapping data for chromosome 17 subdivided into 26 bins, by using a panel of chromosome 17 deletion somatic cell hybrids. One hundred four short tandem repeat and STS markers have been localized into these bins and have enabled the ordering of 288 ESTs and 26 genes, including 142 ESTs that had not been previously sublocalized on chromosome 17. The mapping information of several genetic maps, as well as information obtained by radiation hybrid and STS content mapping of YACs, has been integrated using this hybrid panel. Although existing mapping information for chromosome 17 was generally consistent for many ESTs previously mapped, the map presented here further refines the location of ESTs, as well as demonstrating a number of discrepancies found in the 17q24–q25 region. We attribute these discrepancies to the fact that the current radiation hybrid panels were selected for retention of the thymidine kinase gene at 17q25, as well as to a low concentration of YAC contigs in this region. These data illustrate the benefit of combining multiple mapping techniques to obtain the greatest accuracy. The integration of maps developed by different methods will generate the most accurate genome maps, which may then be used for the generation of large insert clone contigs for chromosome sequencing. Additionally, accurate transcript maps generated by ESTs will greatly speed the isolation of genes linked to disease loci.