Conserved organization of centromeric chromatin in flies and humans
Conserved organization of centromeric chromatin in flies and humans
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DOI：
10.1016/s1534-5807(02)00135-1
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发表时间：
2002-03-01
影响因子：
11.8
通讯作者：
Karpen, GH
中科院分区：
文献类型：
--
作者：
Blower, MD;Sullivan, BA;Karpen, GH
最近的研究强调了着丝粒特异性组蛋白 H3 样 (CENP-A) 蛋白在着丝粒功能中的重要性。我们发现果蝇 CID 和人类 CENP-A 在中期出现为缺乏组蛋白 H3 的三维结构。然而，CID/CENP-A 和 H3 核小体块线性散布在延伸的染色质纤维上，并且 CID 与多核小体制剂中的 H3 核小体接近。当 CID 被 RNAi 耗尽时，它会被 H3 取代，这证明了着丝粒染色质组织的灵活性。最后，与提出 H3 和 CID/CENP-A 核小体在 S 期不同时间复制的模型相反，我们发现散布的 H3 和 CID/CENP-A 染色质在人类和果蝇的 S 期同时复制。我们提出 CID/CENP-A 和 H3 核小体的独特结构排列将着丝粒染色质呈现到浓缩有丝分裂染色体的极面。
Recent studies have highlighted the importance of centromere-specific histone H3-like (CENP-A) proteins in centromere function. We show that Drosophila CID and human CENP-A appear at metaphase as a three-dimensional structure that lacks histone H3. However, blocks of CID/CENP-A and H3 nucleosomes are linearly interspersed on extended chromatin fibers, and CID is close to H3 nucleosomes in polynucleosomal preparations. When CID is depleted by RNAi, it is replaced by H3, demonstrating flexibility of centromeric chromatin organization. Finally, contrary to models proposing that H3 and CID/CENP-A nucleosomes are replicated at different times in S phase, we show that interspersed H3 and CID/CENP-A chromatin are replicated concurrently during S phase in humans and flies. We propose that the unique structural arrangement of CID/CENP-A and H3 nucleosomes presents centromeric chromatin to the poleward face of the condensing mitotic chromosome.