B-wave of the electroretinogram. A reflection of ON bipolar cell activity.
B-wave of the electroretinogram. A reflection of ON bipolar cell activity.
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电网图的B波。双极细胞活性的反射。
DOI：
10.1085/jgp.93.1.101
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发表时间：
1989-01
影响因子：
3.8
通讯作者：
Slaughter, M M
中科院分区：
文献类型：
--
作者：
Stockton, R A;Slaughter, M M
光诱发视网膜内场电位（视网膜电图，ERG）与细胞外钾通量在两栖动物视网膜同时测量。高选择性药物的应用使我们能够在功能上分离各种类型的视网膜神经元。研究发现：(a) APB（2-氨基-4-磷酸丁酸盐）的应用，先前已被证明可以选择性地消除ON双极细胞的光响应性，导致ERG b波和ON钾通量的损失。(b)相反，据报道，PDA（顺式2,3-哌啶-二羧酸）或KYN（犬尿酸）可抑制OFF双极性、水平和三阶视网膜神经元的光反应，导致ERG d波和OFF钾通量的丧失。然而，b波和ON钾通量保持不变。(c) NMA （N-甲基- dl -天冬氨酸）或GLY（甘氨酸），已报道抑制三阶神经元的反应，不减少b波或d波，也不减少ON或OFF时的钾通量。由此得出结论，ERG的b波是ON双极神经元光诱发去极化的结果。该实验方法得出了两个进一步的结论：(a) d波是在光照终止时OFF双极和/或水平细胞去极化的表达；(b)光诱导的视网膜内（近端）和外（远端）细胞外钾浓度的增加是ON双极细胞去极化的结果。
Light-evoked intraretinal field potentials (electroretinogram, ERG) have been measured simultaneously with extracellular potassium fluxes in the amphibian retina. The application of highly selective pharmacologic agents permitted us to functionally isolate various classes of retinal neurons. It was found that: (a) application of APB (2-amino-4-phosphonobutyrate), which has previously been shown to selectively abolish the light responsiveness of ON bipolar cells, causes a concomitant loss of the ERG b-wave and ON potassium flux. (b) Conversely, PDA (cis 2,3-piperidine-dicarboxylic acid) or KYN (kynurenic acid), which have been reported to suppress the light responses of OFF bipolar, horizontal, and third-order retinal neurons, causes a loss of the ERG d-wave as well as OFF potassium fluxes. The b- wave and ON potassium fluxes, however, remain undiminished. (c) NMA (N- methyl-DL-aspartate) or GLY (glycine), which have been reported to suppress the responses of third-order neurons, do not diminish the b- or d-waves, nor the potassium fluxes at ON or OFF. This leads to the conclusion that the b-wave of the ERG is a result of the light-evoked depolarization of the ON bipolar neurons. This experimental approach has resulted in two further conclusions: (a) that the d-wave is an expression of OFF bipolar and/or horizontal cell depolarization at the termination of illumination and (b) that light-induced increases in extracellular potassium concentration in both the inner (proximal) and outer (distal) retina are the result of ON bipolar cell depolarization.