Preventive Acupuncture Intervenes Against Cell Proliferation in 7,12-Dimethylbenz(a)Anthracene-Induced Breast Cancer Model Rats Via the PTEN-PI3K/AKT Signaling Pathway
Preventive Acupuncture Intervenes Against Cell Proliferation in 7,12-Dimethylbenz(a)Anthracene-Induced Breast Cancer Model Rats Via the PTEN-PI3K/AKT Signaling Pathway
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预防性针灸通过 PTEN-PI3K/AKT 信号通路干预 7,12-二甲基苯并(a)蒽诱导的乳腺癌模型大鼠的细胞增殖
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发表时间：
2021
期刊：
影响因子：
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通讯作者：
Rong Zhao
中科院分区：
文献类型：
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作者：
Qinzuo Dong;Xiang Li;Yuhuan Sun;Jun Yang;Ying Chen;Chunlin Xia;Liwei Xing;Yue Xing;Mei Xu;Chunyan Li;Hongmin Wang;Rong Zhao
针灸作为一种安全有效的中医替代疗法，在临床乳腺癌预防领域显示出独特的优势，取得了一定的疗效。然而，关于针刺对乳腺癌形成过程的作用机制缺乏实验证据。用DMBA 100 mg/kg体重灌胃诱发6周龄雌性SD大鼠自发性乳腺癌。预防性针灸治疗。从DMBA给药后第二周开始，每周进行三次，持续16周。他莫昔芬（3毫克/公斤体重，IG，qd）开始后的第一天DMBA管理和持续16周。作为阳性对照治疗。治疗结束后，观察肿瘤重量变化，并进行PI 3 K/Akt信号通路相关蛋白和增殖指数的免疫组化分析。PA和三苯氧胺治疗组与模型组相比，平均瘤重明显降低（P<0.01）。免疫组化结果显示PA组和三苯氧胺组大鼠肿瘤组织中Ki 67和p-AKT的表达明显低于模型组（P<0.01），而PTEN的表达明显高于模型组（P<0.01）。预防性针刺疗法可上调乳腺肿瘤中PTEN的表达，调节PI 3 K/Akt信号通路相关蛋白，抑制增殖指数Ki 67的表达，从而对DMBA诱导的雌性大鼠乳腺肿瘤发生起到抑制作用。
Acupuncture as a safe and effective alternative therapy in traditional Chinese medicine (TCM).which has shown unique advantages as well as achieved efficacy in the field of clinical breast.cancer prevention. However, there is a lack of experimental evidence regarding the.mechanism of action of acupuncture on the process of breast cancer formation. 6-week-old.female SD rats were subjected to a single intragastric administration of DMBA at 100mg/kg.body weight (BW) to induce spontaneous breast cancer. Preventive acupuncture therapy.starting the second week after DMBA administration performed thrice a week for 16 weeks..Tamoxifen (3 mg/kg BW, ig, qd) starting the day right after DMBA administration and lasting.for 16 weeks served as a positive control treatment. After treatment, Tumor weight was.monitored and immunohistochemical analysis of PI3K/Akt Signaling Pathway-related.Proteins and proliferation index of the mammary tumors were performed. Average tumor.weight per rat was significantly decreased in PA and tamoxifen treatment groups compared.with Model group (P＜0.01). Further immunohistochemical analysis revealed that expression.of Ki67 and p-AKT in tumors from rats treated with PA or tamoxifen were significantly lowerthan those from Model group（P＜0.01）while expression of PTEN in tumors from rats treated.with PA or tamoxifen were significantly higher than those from Model group（P＜0.01)..Preventive acupuncture therapy can upregulation the expression of PTEN, regulate the.PI3K/Akt signaling pathway-related proteins, inhibit the expression of proliferation index.Ki67 in mammary tumors, so as to exert an inhibitory effect on DMBA-induced mammary.tumorigenesis in female rats..