Fast Two-Dimensional NMR Spectroscopy of High Molecular Weight Protein Assemblies
Fast Two-Dimensional NMR Spectroscopy of High Molecular Weight Protein Assemblies
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DOI：
10.1021/ja809880p
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发表时间：
2009-03-18
影响因子：
15
通讯作者：
Boisbouvier, Jerome
中科院分区：
文献类型：
--
作者：
Amero, Carlos;Schanda, Paul;Boisbouvier, Jerome
开发了一种结合了甲基-TROSY 和 SOFAST-HMQC 优点的优化 NMR 实验。它可以记录高质量的甲基。几秒钟内数百 kDa 的蛋白质组装体的 H-1-C-13 相关光谱。基于 SOFAST-methyl-TROSY 的实验为研究分子纳米机器在体外发挥生物学功能时发生的结构和动态变化提供了全新的机会。
An optimized NMR experiment that combines the advantages of methyl-TROSY and SOFAST-HMQC has been developed. It allows the recording of high quality methyl. H-1-C-13 correlation spectra of protein assemblies of several hundreds of kDa in a few seconds. The SOFAST-methyl-TROSY-based experiment offers completely new opportunities for the study of structural and dynamic changes occurring in molecular nanomachines while they perform their biological function in vitro.