In vitro colchicine-induced polyploid plantlet production and regeneration from leaf explants of the diploid pear (Pyrus communis L.) cultivar, 'Fertility'
In vitro colchicine-induced polyploid plantlet production and regeneration from leaf explants of the diploid pear (Pyrus communis L.) cultivar, 'Fertility'
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DOI：
10.1080/14620316.2009.11512564
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发表时间：
2009-09-01
影响因子：
1.9
通讯作者：
Bell, Richard L.
中科院分区：
文献类型：
--
作者：
Sun, Qingrong;Sun, Hongyan;Bell, Richard L.
以欧洲梨（Pyrus communis L.）通过离体秋水仙素处理叶片外植体对栽培品种'Fertility'进行了研究。将叶外植体在0.4%（w/v）秋水仙素中孵育24、48或72小时，然后转移到不定芽诱导培养基中。当与来自未处理的芽增殖培养物的对照芽相比时，根据它们的形态特征预先选择再生芽。保持并增殖了具有假定多倍体形态特征的芽。通过流式细胞仪分析所有推测的多倍体个体的倍性水平，并通过茎尖组织压片的染色体计数进行鉴定。使多倍体芽生根，并将所得小植株转移到田间。多倍体植株比二倍体植株的比叶质量高，气孔器大。
Polyploid plantlets, including triploid, tetraploid, and mixoploid, were induced from the European pear (Pyrus communis L.) cultivar 'Fertility' by in vitro colchicine treatment of leaf explants. The leaf explants were incubated in 0.4% (w/v) colchicine for 24, 48, or 72 h, then transferred to adventitious shoot-induction medium. Regenerated shoots were pre-selected according to their morphological characteristics when compared to control shoots from untreated shoot proliferation cultures. Shoots with putative polyploid morphological characteristics were maintained and proliferated. The ploidy levels of all putative polyploid individuals were analysed by flow cytometry and identified by chromosome counts of shoot tip tissue squashes. Polyploid shoots were rooted, and the resulting plantlets were transferred to the field. Polyploid plantlets had a higher specific leaf mass and larger stomata than those of diploid plantlets.