Transfection of luciferase DNA into various cells by cationic cyclodextrin polyrotaxanes derived from ionene-11
Transfection of luciferase DNA into various cells by cationic cyclodextrin polyrotaxanes derived from ionene-11
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DOI：
10.1039/c2jm16425f
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发表时间：
2012-01-01
影响因子：
--
通讯作者：
Wenz, Gerhard
中科院分区：
文献类型：
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作者：
Albuzat, Thomas;Keil, Manuel;Wenz, Gerhard
通过将胺功能环糊精（CDs）缠绕在聚烯核上，制备了用于基因传递的阳离子聚轮烷。这些聚合物被用来在较低的N: P比下将核酸凝聚成1:1的多电解质复合物和与部分未凝聚DNA的复合物。用荧光素酶报告基因转染3个代表性细胞系的实验表明，基于六亚胺- cd的聚轮烷是一种有效的核酸递送剂，诱导的转基因表达水平与聚亚胺（PEI）相当，甚至更高。此外，根据MTT代谢活性测定，三种细胞系对聚轮烷的耐受性都优于PEI。这些数据表明，这种类型的聚轮烷在体内和体外都是很有前途的基因递送剂。
Cationic polyrotaxanes for gene delivery have been generated by threading amine-functional cyclodextrins (CDs) onto polyionene cores. These polymers were used to condense nucleic acids into polyelectrolyte complexes of 1 : 1 and complexes with partly uncondensed DNA at lower N : P ratios. Transfection assays using a luciferase reporter gene in three representative cell lines indicated that a hexamino-CD-based polyrotaxane was an effective nucleic acid delivery agent, inducing comparable and even superior transgene expression levels as poly(ethyleneimine) (PEI). Furthermore, the polyrotaxanes were better tolerated by all three cell lines than PEI as reported by the MTT metabolic activity assay. These data indicate that polyrotaxanes of this type are promising gene delivery agents both in vitro and in vivo.