In vitro organogenesis of gut‐like structures from mouse embryonic stem cells
In vitro organogenesis of gut‐like structures from mouse embryonic stem cells
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DOI：
10.1111/j.1743-3150.2004.00468.x
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发表时间：
2004-04
影响因子：
3.5
通讯作者：
M. Kuwahara;Takunori Ogaeri;Rie Matsuura;Hiroshi Kogo;Toyoshi Fujimoto;S. Torihashi
中科院分区：
文献类型：
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作者：
M. Kuwahara;Takunori Ogaeri;Rie Matsuura;Hiroshi Kogo;Toyoshi Fujimoto;S. Torihashi
胚胎干细胞（ES）具有多能性，并产生许多细胞类型和组织，包括胚胎中所有三个胚层的代表。我们以前曾报道过小鼠ES细胞从胚状体（EB）形成收缩肠样器官。这些肠样结构自发收缩，并具有由三层（即上皮、固有层和肌层）包围的大管腔。周围沿着散在分布有神经节，平滑肌细胞间分布有Cajal间质细胞（ICC）。在本研究中，为了确定它们是否可以作为肠道器官发生的模型，我们研究了与胚胎肠道发育相比的肠道样结构的形成过程。因此，我们发现体外形成的基本过程与体内胚胎肠道发育相似。结果表明，肠样结构不仅是一个有用的工具，用于发育研究，以确定诱导肠道器官发生的因素，而且还用于肠神经元和ICC发育的研究。
Embryonic stem (ES) cells have pluripotency and give rise to many cell types and tissues, including representatives of all three germ layers in the embryo. We have reported previously that mouse ES cells formed contracting gut‐like organs from embryoid bodies (EBs). These gut‐like structures contracted spontaneously, and had large lumens surrounded by three layers, i.e. epithelium, lamina propria and muscularis. Ganglia were scattered along the periphery, and interstitial cells of Cajal (ICC) were distributed among the smooth muscle cells. In the present study, to determine whether they can be a model of gut organogenesis, we investigated the formation process of the gut‐like structures in comparison with embryonic gut development. As a result, we found that the fundamental process of formation in vitro was similar to embryonic gut development in vivo. The result indicates that the gut‐like structure is a useful tool not only for developmental study to determine the factors that induce gut organogenesis, but also for studies of enteric neurone and ICC development.