Molecular cloning of a cDNA encoding the porcine type XVII collagen noncollagenous 16 A domain and localization of the domain to the upper part of porcine skin basement membrane zone.
Molecular cloning of a cDNA encoding the porcine type XVII collagen noncollagenous 16 A domain and localization of the domain to the upper part of porcine skin basement membrane zone.
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编码猪 XVII 型胶原非胶原 16 A 结构域的 cDNA 的分子克隆，并将该结构域定位到猪皮肤基底膜区的上部。
DOI：
10.1111/j.1365-3164.2004.00373.x
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发表时间：
2004
期刊：
影响因子：
--
通讯作者：
Chan,LawrenceS
中科院分区：
文献类型：
--
作者：
Xu,Luting;Olivry,Thierry;Chan,LawrenceS
大疱性类天疱疮是由免疫球蛋白（IG）G自身抗体介导的自身免疫性起泡性人类皮肤病，其靶向皮肤基底膜组分XVII型胶原蛋白，一种跨膜蛋白。XVII型胶原也称为BP 180和BPAG 2，是表皮和下层真皮之间连接所必需的细胞外基质成分。除了作为人类疾病大疱性类天疱疮的靶抗原外，XVII型胶原蛋白还被患有类似起泡皮肤病的犬、猫、马和猪患者的自身抗体靶向。此前，酶联免疫吸附试验和蛋白质印迹分析表明，来自大疱性类天疱疮猪的自身抗体识别XVII型胶原蛋白的人NC 16 A结构域。为了促进猪大疱性类天疱疮模型的发展，我们使用逆转录-聚合酶链反应技术分离编码猪XVII型胶原NC 16 A结构域的cDNA。推导的氨基酸序列与人NC 16 A的同源性为61%。针对猪NC 16 A内的20个氨基酸肽产生的抗体将NC 16 A表位定位于猪皮肤基底膜区的上部。我们的数据提供了猪大疱性类天疱疮靶抗原的进一步信息，可能有助于研究人员对这种疾病的进一步研究。
Bullous pemphigoid is an autoimmune blistering human skin disease mediated by immunoglobulin (Ig)G autoantibodies targeting skin basement membrane component type XVII collagen, a transmembrane protein. Also designated BP180 and BPAG2, type XVII collagen is an extracellular matrix element essential for the connection between the epidermis and the underlying dermis. In addition to being a target antigen in the human disease bullous pemphigoid, type XVII collagen is also targeted by autoantibodies of canine, feline, equine and porcine patients suffering from a similar blistering skin disease. Previously, enzyme‐linked imunosorbent assay and Western blot analyses have shown that autoantibodies from pigs affected with bullous pemphigoid recognize the human NC16A domain of type XVII collagen. To facilitate the development of porcine model of bullous pemphigoid, we isolated cDNA encoding the porcine type XVII collagen NC16A domain using a reverse transcription–polymerase chain reaction technique. The amino acids deduced from the NC16A cDNA showed 61% identity with the sequence of human NC16A. An antibody generated against a 20‐amino acid peptide within the porcine NC16A localized the NC16A epitope to the upper part of porcine skin basement membrane zone. Our data provide further information of the porcine bullous pemphigoid target antigen and may help investigators for their further studies of this disease.