Microarray gene expression profiling of developmental transitions in Sitka spruce (Picea sitchensis) apical shoots
Microarray gene expression profiling of developmental transitions in Sitka spruce (Picea sitchensis) apical shoots
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DOI：
10.1093/jxb/erl246
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发表时间：
2007-02-01
影响因子：
6.9
通讯作者：
Douglas, Carl J.
中科院分区：
文献类型：
--
作者：
Friedmann, Michael;Ralph, Steven G.;Douglas, Carl J.
顶芽驱动针叶树每年的新茎生长，是建立化学和物理防御的主要场所，并且在建立随后的多年生植物中很重要。这个器官提出了一个有趣的发育系统，生长和发育进展从一个分生组织尖端通过发展的初级维管系统，一个基地与完全分化和木质化的次生木质部的内部和树皮组织与组成防御结构，如树脂，多酚韧皮薄壁细胞，和石细胞的外部。云杉（Picea spp.）利用含有约16.7K个独特cDNA的基因芯片研究了锡特卡云杉（Piceasitchensis）顶芽从营养顶端到木质基部发育转变的转录谱。沿着细胞壁修饰和木质素生物合成相关基因的发现，还发现了一系列具有碱基优先表达模式的蛋白激酶和转录因子基因，这些基因可能在顶芽木质组织的形成以及其他与器官成熟相关的发育转变中发挥作用。已知的针叶树防御基因，基因编码的防御相关蛋白，和基因编码的调控蛋白的优先表达被观察到在顶端梢尖和在绿色树皮组织在顶端梢基地，这表明一个承诺，组成防御在顶端梢，是协调与快速发展的次生木质部。
The apical shoot drives the yearly new stem growth of conifer trees, is the primary site for the establishment of chemical and physical defences, and is important in establishing subsequent perennial growth. This organ presents an interesting developmental system, with growth and development progressing from a meristematic tip through development of a primary vascular system, to a base with fully differentiated and lignified secondary xylem on the inside and bark tissue with constitutive defence structures such as resin, polyphenolic phloem parenchyma cells, and sclereids on the outside. A spruce (Picea spp.) microarray containing approximately 16.7K unique cDNAs was used to study transcript profiles that characterize the developmental transition in apical shoots of Sitka spruce (Picea sitchensis) from their vegetative tips to their woody bases. Along with genes involved in cell-wall modification and lignin biosynthesis, a number of differentially regulated genes encoding protein kinases and transcription factors with base-preferred expression patterns were identified, which could play roles in the formation of woody tissues inside the apical shoot, as well as in regulating other developmental transitions associated with organ maturation. Preferential expression of known conifer defence genes, genes encoding defence-related proteins, and genes encoding regulatory proteins was observed at the apical shoot tip and in the green bark tissues at the apical shoot base, suggesting a commitment to constitutive defence in the apical shoot that is co-ordinated with rapid development of secondary xylem.