Paeoniflorin inhibited the tumor invasion and metastasis in human hepatocellular carcinoma cells
Paeoniflorin inhibited the tumor invasion and metastasis in human hepatocellular carcinoma cells
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芍药苷抑制人肝癌细胞的肿瘤侵袭和转移
DOI：
10.4149/bll_2014_084
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发表时间：
2014-01-01
影响因子：
1.5
通讯作者：
Wei, W.
中科院分区：
文献类型：
--
作者：
Lu, J. T.;He, W.;Wei, W.
目的：有证据表明，芍药苷可能参与抗癌活性。材料与方法：本研究应用四甲基偶氮唑蓝(3-(4，5-二甲基噻唑-2-基)-2，5-二苯基四氮唑溴化法)检测了人肝癌细胞株HepG(2)和Bel-7402的增殖作用，建立了Boyden小室实验、伤口愈合实验和细胞黏附实验来检测和定量检测两种细胞的侵袭、转移和黏附。结果：芍药苷(6.25~200 mU M)对人肝癌细胞株HepG(2)和Bel-7402细胞的生长有抑制作用，并能显著降低肝癌细胞株的侵袭、转移和黏附能力。结论：芍药苷是一种有效的抗转移和抗侵袭剂，能抑制肝癌细胞的侵袭和转移。30)。PDF格式的文本www.elis.sk。
Objective: Evidence suggests that paeoniflorin may be involved in anticancer activities. Here, we have investigated the effects of paeoniflorin and correlative mechanisms on anti-invasion and anti-metastasis in human hepatocellular carcinoma (HCC) cell lines.Materials and methods: In the current study, we have applied 3-(4,5-dimethylthiazol-2-yl)-2, 5-diphenyl tetrazolium bromide (MTT) assay to determine the proliferative effect of HepG(2) and Bel-7402, two human hepatoma cell lines, and we have established a boyden chamber assay, a wound healing assay and cell adhesion assay to detect and quantify the invasion, metastasis and adhesion of both HepG(2) and Bel-7402. In addition, we have analyzed the protein expression of matrix metalloproteinas (MMP)-9, E-cadherin (E-cad) and extracellular signal-regulated kinase (ERK) in both cell lines through western blot analysis.Results: Paeoniflorin (6. 25-200 mu M) had inhibitory effect on the growth of HepG(2) and Bel-7402 cell lines, and reduced significantly invasion, metastasis and adhesion of HCC cell lines. In addition, paeoniflorin decreased the expression of MMP-9 and ERK in HepG(2) and Bel-7402 cells, and increased expression of E-cad in both cell lines.Conclusions: Paeoniflorin is effective anti-metastatic and anti-invasive agent for suppressing HCC invasion and metastasis (Fig. 5, Ref. 30). Text in PDF www.elis.sk.