Retinoid-related orphan nuclear receptor RORβ is an early-acting factor in rod photoreceptor development
Retinoid-related orphan nuclear receptor RORβ is an early-acting factor in rod photoreceptor development
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DOI：
10.1073/pnas.0902425106
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发表时间：
2009-10-13
影响因子：
11.1
通讯作者：
Forrest, Douglas
中科院分区：
文献类型：
--
作者：
Jia, Li;Oh, Edwin C. T.;Forrest, Douglas
视杆细胞和视锥细胞是形态和发育上不同的光感受器类型，在视觉中具有不同的功能。视锥细胞介导日光和色觉，在大多数哺乳动物中，视锥细胞表达M和S视蛋白色素，分别对中长和短光波长敏感。视杆细胞介导弱光视觉并表达视紫红质色素。指导每种光感受器类型分化的转录因子网络并不完全确定。在这里，我们报告说，Rorb（-/-）小鼠缺乏维甲酸相关的孤儿核受体β失去杆，但过度产生原始的S锥，缺乏外段。表型反映了视杆细胞和视锥细胞谱系之间明显的可塑性，并且与缺乏神经视网膜亮氨酸拉链因子的Nrl（-/-）小鼠所描述的相似。Rorb（-/-）小鼠缺乏Nrl表达，并且Rorb（-/-）小鼠中Nrl的再表达将视锥细胞转化为杆状细胞。因此，Rorb指导视杆发育，并且至少部分地通过诱导Nrl介导的视杆分化途径来这样做。
Rods and cones are morphologically and developmentally distinct photoreceptor types with different functions in vision. Cones mediate daylight and color vision and in most mammals express M and S opsin photopigments for sensitivity to medium-long and short light wavelengths, respectively. Rods mediate dim light vision and express rhodopsin photopigment. The transcription factor networks that direct differentiation of each photoreceptor type are incompletely defined. Here, we report that Rorb(-/-) mice lacking retinoid-related orphan nuclear receptor beta lose rods but overproduce primitive S cones that lack outer segments. The phenotype reflects pronounced plasticity between rod and cone lineages and resembles that described for Nrl(-/-) mice lacking neural retina leucine zipper factor. Rorb(-/-) mice lack Nrl expression and reexpression of Nrl in Rorb(-/-) mice converts cones to rod-like cells. Thus, Rorb directs rod development and does so at least in part by inducing the Nrl-mediated pathway of rod differentiation.