Inhibition of glomerular glucosaminyl N-deacetylase in diabetic rats.
Inhibition of glomerular glucosaminyl N-deacetylase in diabetic rats.
复制标题
糖尿病大鼠肾小球氨基葡萄糖N-脱乙酰酶的抑制。
DOI：
10.1038/ki.1992.119
复制
发表时间：
1992
影响因子：
19.6
通讯作者：
A. Kofoed‐Enevoldsen
中科院分区：
文献类型：
--
作者：
A. Kofoed‐Enevoldsen
酶葡糖胺基N-脱乙酰基酶在硫酸乙酰肝素生物合成中起关键作用，因为N-脱乙酰基是碳水化合物聚合物的N-和进一步O-硫酸化的先决条件。糖尿病引起的这种酶的抑制可能是糖尿病肾病发展的重要因素。在这项研究中，肾小球氨基葡萄糖N-脱乙酰酶活性和尿白蛋白排泄测定胰岛素治疗链脲佐菌素糖尿病大鼠。此外，在试图提供的N-脱乙酰酶的脆弱性的遗传依赖性差异的证据，两个密切相关的大鼠品系（H和U）进行了研究。在平均血糖值在9和17 mmol/L之间的大鼠中发现酶活性的显著10%抑制，P小于0.05。有一个显着的差异，两种大鼠品系的酶对血糖变化的脆弱性。根据血糖与N-脱乙酰酶活性之间的相关性判断，U大鼠对短期血糖控制高度敏感（r =-0.73，P = 0.005，N = 16），而在H大鼠中没有发现这种相关性（r = 0.02，P = 0.9，N = 14）。糖尿病H组大鼠尿白蛋白排泄量增加，且与肾小球N-脱乙酰酶活性显著相关（r =-0.62，P = 0.02，N = 14）。与H大鼠相比，U大鼠的白蛋白排泄量增加了10倍，但这种白蛋白尿显然与糖尿病的存在无关，也与肾小球N-脱乙酰酶活性无关。结论：糖尿病引起的氨基葡萄糖N-脱乙酰酶抑制可能在糖尿病肾病的发病中起重要作用。
The enzyme glucosaminyl N-deacetylase plays a key role in heparan sulphate biosynthesis since N-deacetylation is a prerequisite for N- and further O-sulphation of the carbohydrate polymer. Diabetes induced inhibition of this enzyme could be an important factor in the development of diabetic nephropathy. In this study glomerular glucosaminyl N-deacetylase activity and urinary albumin excretion were measured in insulin-treated streptozotocin-diabetic rats. Furthermore, in an attempt to provide evidence of genetically dependent differences in the vulnerability of the N-deacetylase enzyme, two closely related rat strains (H and U) were studied. A significant 10% inhibition in enzyme activity was found among rats with mean blood glucose values between 9 and 17 mmol/liter, P less than 0.05. There was a pronounced difference between the two rat strains in the vulnerability of the enzyme against blood glucose alterations. The U rat appeared highly sensitive to short-term blood glucose control judged by the correlation between blood glucose and N-deacetylase activity (r = -0.73, P = 0.005, N = 16), where no such correlation was found in the H rat (r = 0.02, P = 0.9, N = 14). Urinary albumin excretion was increased in diabetic H rats and significantly correlated to glomerular N-deacetylase activity (r = -0.62, P = 0.02, N = 14). The U rats developed a 10-fold rise in albumin excretion compared to H rats, but this albuminuria was apparently not related to the presence of diabetes or correlated to glomerular N-deacetylase activity. It is concluded that the diabetes induced inhibition of glucosaminyl N-deacetylase may play an important role in the pathogenesis of diabetic nephropathy.
肝素和肾小球上皮细胞分泌的肝素样物质抑制系膜细胞增殖。
DOI：
--
发表时间：
1985
期刊：
The American journal of pathology
影响因子：
--
作者：
CastellotJr,JJ;Hoover,RL;Harper,PA;Karnovsky,MJ
通讯作者：
Karnovsky,MJ
以大肠杆菌 K5 荚膜多糖为底物测定 N-乙酰乙酰肝素脱乙酰酶。
影响因子：
2.9
作者：
Navia,JL;Riesenfeld,J;Vann,WF;Lindahl,U;Rodén,L
通讯作者：
Rodén,L