Soft-tissue Wound Healing by Anti-advanced Glycation End-products Agents
Soft-tissue Wound Healing by Anti-advanced Glycation End-products Agents
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DOI：
10.1177/0022034514523785
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发表时间：
2014-04-01
影响因子：
7.6
通讯作者：
Chen, C. -C.
中科院分区：
文献类型：
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作者：
Chang, P. -C.;Tsai, S. -C.;Chen, C. -C.
阻断晚期糖基化终末产物 (AGE) 已被证明可以减少糖尿病并发症并控制牙周炎。本研究调查了在施用氨基胍（AG）（一种AGE抑制剂）或N-苯甲酰基溴化噻唑鎓（PTB）（一种糖化交联破坏剂）的情况下移植物收获后腭部伤口愈合的模式。在 72 只 Sprague-Dawley 大鼠中产生全层腭切除伤口 (5.0 x 1.5 mm(2))。大鼠每天腹腔注射生理盐水（对照）、AG 或 PTB，并在 4 至 28 天后处死。通过组织学、胶原基质沉积的组织化学、AGE 和 AGE 受体 (RAGE) 的免疫组织化学、RAGE 的表达以及炎症和恢复相关基因来评估伤口愈合模式。在 AG 或 PTB 治疗后的前 14 天，伤口闭合、上皮再形成和胶原基质沉积加速，而 AGE 沉积、RAGE 阳性细胞和炎症减少。 RAGE和肿瘤坏死因子-α在第7天显着下调，血红素加氧酶-1持续下调直至第14天。血管内皮生长因子、骨膜素、I型胶原和纤连蛋白的水平在第14天均增加。总之，抗AGE药物似乎通过减少AGE相关炎症和促进腭伤口愈合来促进腭伤口愈合。 恢复过程。
The blocking of advanced glycation end-products (AGE) has been shown to reduce diabetic complications and control periodontitis. This study investigated the pattern of palatal wound-healing after graft harvesting under the administration of aminoguanidine (AG), an AGE inhibitor, or N-phenacylthiazolium bromide (PTB), a glycated cross-link breaker. Full-thickness palatal excisional wounds (5.0 x 1.5 mm(2)) were created in 72 Sprague-Dawley rats. The rats received daily intraperitoneal injections of normal saline (control), AG, or PTB and were euthanized after 4 to 28 days. The wound-healing pattern was assessed by histology, histochemistry for collagen matrix deposition, immunohistochemistry for AGE and the AGE receptor (RAGE), and the expression of RAGE, as well as inflammation- and recovery-associated genes. In the first 14 days following AG or PTB treatments, wound closure, re-epithelialization, and collagen matrix deposition were accelerated, whereas AGE deposition, RAGE-positive cells, and inflammation were reduced. RAGE and tumor necrosis factor-alpha were significantly down-regulated at day 7, and heme oxygenase-1 was persistently down-regulated until day 14. The levels of vascular endothelial growth factor, periostin, type I collagen, and fibronectin were all increased at day 14. In conclusion, anti-AGE agents appeared to facilitate palatal wound-healing by reducing AGE-associated inflammation and promoting the recovery process.