Fast Super-Resolution Imaging Technique and Immediate Early Nanostructure Capturing by a Photoconvertible Fluorescent Protein
Fast Super-Resolution Imaging Technique and Immediate Early Nanostructure Capturing by a Photoconvertible Fluorescent Protein
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快速超分辨率成像技术和通过光转换荧光蛋白立即捕获早期纳米结构
DOI：
10.1021/acs.nanolett.9b02855
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发表时间：
2020
期刊：
影响因子：
10.8
通讯作者：
Xu Pingyong
中科院分区：
文献类型：
--
作者：
Zhang Mingshu;Fu Zhifei;Li Changqing;Liu Anyuan;Peng Dingming;Xue Fudong;He Wenting;Gao Shan;Xu Fan;Xu Dan;Yuan Ling;Zhang Fa;Xu Zhiheng;Xu Tao;Xu Pingyong
低时间分辨率和有限的光控荧光蛋白探针限制了单分子定位显微镜（SMLM）的广泛应用。在当前的研究中，我们开发了一种新的光转换荧光蛋白（PCFP）、pcStar和快速单分子引导贝叶斯定位显微镜（Quick-SIMBA）。 pcStar和Quick-SIMBA的结合实现了SMLM方法中最高的时间分辨率（0.1-0.25 s）和大视场（76 × 9.4 μm2−76 × 31.4 μm2），从而能够解析内质网致密管状基质的动态运动。此外，pcStar将SMLM的应用扩展到果蝇胚胎的早期纳米结构成像，并揭示了神经元-胶质细胞连接处的特定“平行三柱”结构，有助于阐明果蝇胚胎发生过程中胶质细胞对神经元的“锁定”和支持。
Low temporal resolution and limited photocontrollable fluorescent protein probes have restricted the widespread application of single-molecule localization microscopy (SMLM). In the current study, we developed a new photoconvertible fluorescent protein (PCFP), pcStar, and quick single molecule-guided Bayesian localization microscopy (Quick-SIMBA). The combination of pcStar and Quick-SIMBA achieved the highest temporal resolution (0.1–0.25 s) with large field-of-view (76 × 9.4 μm2−76 × 31.4 μm2) among the SMLM methods, which enabled the dynamic movements of the endoplasmic reticulum dense tubular matrix to be resolved. Moreover, pcStar extended the application of SMLM to imaging the immediate early nanostructures inDrosophilaembryos and revealed a specific “parallel three-pillar” structure in the neuronal-glial cell junction, helping to elucidate glial cell “locking” and support of neurons duringDrosophilaembryogenesis.