Effect of regenerated endothelium on lipid accumulation in the arterial wall.
Effect of regenerated endothelium on lipid accumulation in the arterial wall.
复制标题
再生内皮对动脉壁脂质积累的影响。
DOI：
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发表时间：
1977
影响因子：
11.1
通讯作者：
William Insull
中科院分区：
文献类型：
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作者：
C. Minick;M. B. Stemerman;William Insull
我们验证了这样一种假设，即内皮细胞的丧失会导致脂蛋白向动脉壁的运输增加，有利于脂质的积聚，从而易于发生动脉粥样硬化。在最初喂食低脂饮食的兔子中，用动脉内球囊导管去内皮化主动脉；28天后，动物被分成两组。I组动物继续低脂饮食，分别于去内皮化后8、11、13和15周处死。第二组动物饲喂添加0.5%胆固醇的相同日粮，并以相同的间隔处死。I组动物的主动脉内膜去内皮化区和再内皮化区均表现为增生性纤维肌层增厚，内膜脂肪改变很少或没有。相比之下，II组动物的主动脉内膜有轻微到明显的脂肪改变，其特征是油红O阳性物质积聚，并伴有各向异性的脂质包裹体。脂质含量最多的是再生内皮下的内膜增厚，而不是邻近无内皮内膜的内膜增厚。这些结果不支持内皮缺失有利于脂质堆积和易患动脉粥样硬化的假说。实验表明，脂质优先积聚在新生内皮覆盖的血管内膜增厚区域。
We tested the hypothesis that loss of endothelium results in increased transport of lipoprotein into the arterial wall, favors accumulation of lipid, and thus predisposes to atherosclerosis. In rabbits initially fed a diet low in lipid, the aortas were de-endothlialized with an intraarterial balloon catheter; 28 days later, the animals were divided into two groups. Group I animals were continued on a diet low in lipid and sacrificed at 8, 11, 13, and 15 weeks after de-endothelialization. Group II animals were fed the same diet supplemented with 0.5% cholesterol and sacrificed at comparable intervals. Aortas of group I animals revealed proliferative fibromuscular intimal thickening in both de-endothelialized and re-endothelialized areas, with little or no fatty change in the intima. In contrast, aortas of group II animals revealed slight to marked fatty change in the intima, characterized by accumulation of oil red O-positive material with anisotropic lipid inclusions. The greatest quantity of lipid was present in intimal thickening beneath regenerated endothelium, and not in adjacent intimal thickening lacking an endothelial lining. These results do not support the hypothesis that the absence of endothelium favors accumulation of lipid and predisposes to atherosclerosis. The experiments indicate that lipid accumulates preferentially in areas of intimal thickening covered by regenerated endothelium.