Physical characteristics in eucaryotic promoters
Physical characteristics in eucaryotic promoters
复制标题
真核启动子的物理特性
DOI：
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发表时间：
1983
期刊：
影响因子：
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通讯作者：
C. Reiss
中科院分区：
文献类型：
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作者：
M. Bensimhon;J. Gabarro;R. Ehrlich;C. Reiss
对于一系列野生型和突变的真核基因前导序列(主要是SV40早期基因的启动子)(Benoist和Chambon，Nature 290,304(1981)；Moreau等，Nuc.酸结果9,6047(1982))和单纯疱疹病毒TK基因(McKnight和Kingsbury，Science 217,316(1982))，体内启动子活性和局部稳定性(变性)已被关联。与这些论文得出的结论一致，相关性指向三个主要的真核启动子元件和基因座：(I)由增强子序列激活的酶；SV40和Moloney肉瘤病毒增强子具有显著的稳定性同源性；(Ii)酶激活，发生在50-70b.p。该酶位于帽子上游的一个高稳定区域；该酶在远离TRAP位点时明显呈指数级失活；(Iii)TRAP位点的酶定位为30+/-5b.p。在盖子场地的上游。陷阱位置包含TATA盒，或者，如果没有TATA盒，则包含激活剂下游的其他低稳定域。帽部位的数量和占有率可能取决于陷阱部位-帽部位对的稳定性和大小以及它与激活剂的距离。
For a series of wild type and mutated eucaryotic gene prelude sequences (mainly "promoters" of SV40 early gene (Benoist and Chambon, Nature 290, 304 (1981); Moreau et al., Nuc. Acids Res. 9, 6047 (1982)) and of Herpes Simplex Virus TK gene (McKnight and Kingsbury, Science 217, 316 (1982)), in vivo promoter activity and local stability (denaturability) have been correlated. In agreement with the conclusions drawn in these papers, the correlation points to three major eucaryotic promoter elements and loci: (i) enzyme enabling by an enhancer sequence; SV40 and Moloney Sarcoma Virus enhancers have a striking stability homology; (ii) enzyme activation, occurring 50-70 b.p. upstream the cap site in a high stability domain; the enzyme apparently deactivates exponentially upon moving away to trap site; (iii) enzyme positioning at trap site, 30 +/- 5 b.p. upstream the cap site. The trap site contains the TATA box, or, when absent, other low stability domains downstream the activator. The number and occupancy of cap sites may depend on the stability and size of the trap site-cap site couple and its distance from the activator.