Transcription organization and mRNA levels of the genes for all 12 subunits of the fission yeast RNA polymerase II
Transcription organization and mRNA levels of the genes for all 12 subunits of the fission yeast RNA polymerase II
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裂殖酵母 RNA 聚合酶 II 所有 12 个亚基基因的转录组织和 mRNA 水平
DOI：
10.1046/j.1365-2443.2001.00394.x
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发表时间：
2001
期刊：
影响因子：
2.1
通讯作者：
A. Ishihama
中科院分区：
文献类型：
--
作者：
H. Sakurai;A. Ishihama
真核生物的RNA聚合酶II（Pol II）由12个亚基组成，其中5个亚基由Pol I、Pol II和Pol III共享。然而，目前对12个Pol II亚基的合成和组装的调控知之甚少。为了深入了解这12个Pol II亚基，Rpb 1至Rpb 12，在裂殖酵母裂殖酵母中的合成调控，我们分析了rpb基因的转录组织，通过使用寡核苷酸加帽方法，并通过定量竞争PCR测定mRNA水平。
The RNA polymerase II (Pol II) of eukaryotes is composed of 12 subunits, of which five are shared among Pol I, Pol II and Pol III. At present, however, little is known about the regulation of synthesis and assembly of the 12 Pol II subunits. To obtain an insight into the regulation of synthesis of these 12 Pol II subunits, Rpb1 to Rpb12, in the fission yeast Schizosaccharomyces pombe, we analysed the transcriptional organization of the rpb genes by use of the oligo capping method, and determined mRNA levels by quantitative competitive PCR assay.
影响因子：
56.9
作者：
Cramer, P;Bushnell, DA;Kornberg, RD
通讯作者：
Kornberg, RD
粟酒裂殖酵母的丙糖磷酸异构酶基因的转录起始点与酿酒酵母不同。
影响因子：
3.5
作者：
Russell,PR
通讯作者：
Russell,PR