KINETICS OF MAST-CELL, BASOPHIL AND EOSINOPHIL POPULATIONS AT HYALOMMA-ANATOLICUM-ANATOLICUM FEEDING SITES ON CATTLE AND THE ACQUISITION OF RESISTANCE
KINETICS OF MAST-CELL, BASOPHIL AND EOSINOPHIL POPULATIONS AT HYALOMMA-ANATOLICUM-ANATOLICUM FEEDING SITES ON CATTLE AND THE ACQUISITION OF RESISTANCE
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DOI：
10.1017/s0031182000051477
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发表时间：
1986-10-01
期刊：
影响因子：
2.4
通讯作者：
GILL, HS
中科院分区：
文献类型：
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作者：
GILL, HS
通过比较在H. a. anatolicum喂养网站后，第一次和第三次侵扰。牛对H. a. anatolicum喂养后，一个单一的侵扰。抗性表现为饱食雌性的平均重量和产生的卵质量的平均重量显著降低，不能产卵，有时蜱原位死亡。获得的阻力对应于明显的表皮水泡和一个显着的变化，在蜱虫喂养部位的细胞浸润的性质，表明皮肤超敏反应的发展。在原发性感染时，细胞浸润主要为中性粒细胞（43-71%），其次为单核细胞（25-35%）。分别在初次侵染后72和144 h首次观察到少量嗜碱性粒细胞和嗜酸性粒细胞浸润。与原发性感染不同，在第三次感染后24小时，皮肤浸润的特点是嗜碱性粒细胞（6%）和嗜酸性粒细胞（3%）的浸润和肥大细胞和嗜碱性粒细胞的明显脱粒。嗜碱性粒细胞的绝对值和相对值随着摄食的增加而增加。在72 h（16%）和144 h（23%）时，它们是仅次于中性粒细胞（48-68%）的第二大丰度细胞类型。嗜酸性粒细胞（3-9%）和单核细胞的平均数量也显示出显着增加，与原发性感染相比。蜱虫侵染后可检测到的肥大细胞数量逐渐减少，与原发性侵染相比，第三次侵染后肥大细胞数量显著降低。这些结果表明，肥大细胞和嗜碱性粒细胞脱颗粒释放的药理学介质可能是牛对H. a. anatolicum。
The cutaneous cellular reactions associated with acquisition of resistance by cattle to Hyalomma anatolicum anatolicum feeding were investigated by comparing the nature and sequence of cellular events at H. a. anatolicum feeding sites following primary and tertiary infestations. Cattle acquired resistance to H. a. anatolicum feeding after a single infestation. The resistance was manifested by a significant reduction in the mean weight of engorged females and mean weight of egg mass produced, failure to lay eggs and sometimes death of ticks in situ. The acquisition of resistance corresponded with pronounced epidermal vesiculation and a significant change in the nature of the cellular infiltrate at tick feeding sites indicating the development of cutaneous hypersensitivity. On primary infestation the cellular infiltrate was dominated by neutrophils (43-71%) followed by mononuclear cells (25-35%). Basophil and eosinophil infiltration in small numbers was noticed for the first time at 72 and 144 h, respectively, post-primary infestation. Unlike primary infestation, the dermal infiltrate on tertiary infestation, 24 h after attachment, was characterized by infiltration of basophils (6%) and eosinophils (3%) and a marked degranulation of mast cells and basophils. Basophils showed a consistent increase in absolute as well as relative numbers as the feeding advanced. They were the second most abundant cell types after neutrophils (48-68%) at 72 h (16%), and 144 h (23%). Eosinophils (3-9%) and mononuclear cells also showed a significant increase in their mean numbers as compared with primary infestation. There was a progressive decrease in the number of detectable mast cells following tick infestation and they were significantly lower post-tertiary infestation, compared to primary infestation. These results suggest that the pharmacological mediators released by degranulation of mast cells and basophils are perhaps the major effectors of resistance in cattle to H. a. anatolicum.