Ziwi, the zebrafish homologue of the Drosophila piwi:: co-localization with vasa at the embryonic genital ridge and gonad-specific expression in the adults
Ziwi, the zebrafish homologue of the Drosophila piwi:: co-localization with vasa at the embryonic genital ridge and gonad-specific expression in the adults
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DOI：
10.1016/s0925-4773(03)00120-5
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发表时间：
2002-12-01
影响因子：
2.6
通讯作者：
Gong, Zhiyuan
中科院分区：
文献类型：
--
作者：
Tan, Cheong-Huat;Lee, Thean-Chu;Gong, Zhiyuan
PIWI调节多种生物中生殖系干细胞的增殖和维持。全长3.26 kb的ziwi cDNA是果蝇piwi的斑马鱼同源物，编码一个含858个氨基酸的假定蛋白质。ZIWI与小鼠和人类的PIWI有65%的同源性，但与秀丽隐杆线虫和果蝇的PIWI分别只有38%和33%的同源性。在成年斑马鱼中，ziwi仅在性腺中表达。在胚胎和鱼苗中，其表达在体节形成期最初可检测到，并在孵化后至少持续4周。在神经发生和器官形成过程中，在中枢神经系统和鳍芽中检测到其表达。从受精后24小时开始及之后，在生殖嵴中发现了ziwi转录本。（c）2003爱思唯尔科学爱尔兰有限公司。保留所有权利。
PIWI regulates the proliferation and maintenance of germline stem cells in diverse organisms. The full-length 3.26 kb ziwi cDNA, the zebrafish homologue of piwi of Drosophila, encodes a putative protein of 858 amino acids. ZIWI is 65% homologous with the mouse and human PIWI, but only 38 and 33% with Caenorhabditis elegans and Drosophila PIWI, respectively. In adult zebrafish, ziwi is expressed exclusively in the gonads. In embryos and fry, its expression is detectable initially during segmentation and persisted for at least 4 weeks post hatching. During neurogenesis and organogenesis, its expression was detected in the CNS and fin buds. Starting from 24 hpf and later on, ziwi transcripts were found in the genital ridge. (c) 2003 Elsevier Science Ireland Ltd. All rights reserved.