Cyclic changes in metabolic state during the life of a yeast cell
Cyclic changes in metabolic state during the life of a yeast cell
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DOI：
10.1073/pnas.0708365104
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发表时间：
2007-10-23
影响因子：
11.1
通讯作者：
McKnight, Steven L.
中科院分区：
文献类型：
--
作者：
Tu, Benjamin P.;Mohler, Rachel E.;McKnight, Steven L.
在营养有限的环境中，出芽酵母在连续生长过程中经历了强劲的氧气消耗振荡。利用液相色谱-质谱法和综合二维气相色谱-质谱法的代谢物分析方法，我们确定了许多代谢物的细胞内浓度作为这些代谢循环的函数周期性变化。这些结果揭示了酵母细胞在不同生命阶段的细胞代谢逻辑。它们可能进一步表明，关键代谢物丰度的振荡可能有助于控制细胞过程的时间调节和周期的建立。这种代谢状态的振荡可能发生在其他生物周期过程中。
Budding yeast undergo robust oscillations in oxygen consumption during continuous growth in a nutrient-limited environment. Using liquid chromatography-mass spectrometry and comprehensive 2D gas chromatography-mass spectrometry-based metabolite profiling methods, we have determined that the intracellular concentrations of many metabolites change periodically as a function of these metabolic cycles. These results reveal the logic of cellular metabolism during different phases of the life of a yeast cell. They may further indicate that oscillation in the abundance of key metabolites might help control the temporal regulation of cellular processes and the establishment of a cycle. Such oscillations in metabolic state might occur during the course of other biological cycles.