An equibiaxial strain system for cultured cells
An equibiaxial strain system for cultured cells
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DOI：
10.1152/ajpcell.1996.271.4.c1400
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发表时间：
1996-10-01
影响因子：
5.5
通讯作者：
McCulloch, AD
中科院分区：
文献类型：
--
作者：
Lee, AA;Delhaas, T;McCulloch, AD
我们开发了一种装置，该装置将0-10%的均匀等双轴应变施加到细胞培养基底上，并定量验证基底变形对培养的心肌细胞的传递。在单井和多井版本的装置中的夹紧弹性膜通过用塑料环压痕而均匀拉伸，从而产生与节距半径比成正比的应变。通过跟踪附着在基底上的荧光微球和培养的成年大鼠心脏成纤维细胞来测量二维变形。对于高达18%的标称拉伸，沿周向和径向轴的应变沿着大小相等，且均匀分布，剪切力可忽略不计。对于5%拉伸，基底中的周向和径向应变分别为0.046 +/- 0.005和0.048 +/- 0.004 [不显著（NS）]，剪切应变为0.001 +/- 0.003（NS）。单井和多孔版本的校准允许通过设备旋转进行菌株选择。在培养的细胞中均匀的等双轴应变的可重复的应用和定量提供了一种将机械刺激与细胞转导机制相关联的定量方法。
We developed a device that applies homogeneous equibiaxial strains of 0-10% to a cell culture substrate and quantitatively verified transmission of substrate deformation to cultured cardiac cells. Clamped elastic membranes in both single-well and multiwell versions of the device are uniformly stretched by indentation with a plastic ring, resulting in strain that is directly proportional to the pitch-to-radius ratio. Two-dimensional deformations were measured by tracking fluorescent microspheres attached to the substrate and to cultured adult rat cardiac fibroblasts. For nominal stretches up to 18%, strains along circumferential and radial axes were equal in magnitude and homogeneously distributed with negligible shear. For 5% stretch, circumferential and radial strains in the substrate were 0.046 +/- 0.005 and 0.048 +/- 0.004 [not significant (NS)], respectively, and shear strain was 0.001 +/- 0.003 (NS). Calibration of both single-well and multiwell versions permits strain selection by device rotation. The reproducible application and quantification of homogeneous equibiaxial strain in cultured cells provides a quantitative approach for correlating mechanical stimuli to cellular transduction mechanisms.