Ultrafast infrared chemical imaging of live cells
Ultrafast infrared chemical imaging of live cells
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DOI：
10.1039/c0sc00409j
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发表时间：
2011-01-01
期刊：
影响因子：
8.4
通讯作者：
Phillips, Chris C.
中科院分区：
文献类型：
--
作者：
Amrania, Hemmel;McCrow, Andrew P.;Phillips, Chris C.
中红外光谱提供了生物材料（包括DNA和蛋白质）的独特化学指纹，从单个分子到高度组织化的结构，最终到活细胞和组织。然而，在细胞水平上获得良好的信噪比中红外光谱图像通常涉及同步加速器，成像时间为分钟级。在这里，我们使用一种新的基于激光的台式红外光谱显微成像系统，以衍射有限的空间分辨率获得活的人类卵巢癌细胞的振动指纹特征，并且光谱分辨率（<20 cm（-1））足以绘制出细胞内部化学部分的空间分布。明亮的激光脉冲给出非常高的信噪比图像，并且类似于100皮秒的图像采集时间，比当前的中红外光谱成像技术快大约10（11）倍。该成像方法是定量的，无光毒性，无标记物，并且足够快地“冻结”移动的活标本。它可以应用于一系列细胞水平的生化过程，我们相信它可以影响药物作用，细胞生理学，病理学和疾病作为一个整体的领域。
Mid-infrared (mid-IR) spectroscopy provides a unique chemical fingerprint of biomaterials, including DNA and proteins, from single molecules to highly organised structures and, ultimately, to live cells and tissues. However, acquiring good signal-to-noise mid-IR spectroscopic images, at the cellular level, typically involves a synchrotron, with imaging times of order of minutes. Here we use a new laser-based table-top IR spectroscopic micro-imaging system, to obtain vibrational fingerprint signatures of living human ovarian cancer cells at a diffraction limited spatial resolution, and at a spectral resolution (< 20 cm(-1)) sufficient to map out the spatial distributions of chemical moieties inside the cell itself. The bright laser pulses give very high signal-to-noise images, and similar to 100 psec image acquisition times that are roughly 10(11) times faster than current mid-IR spectroscopic imaging techniques. The imaging method is quantitative, non-phototoxic, marker-free and easily fast enough to "freeze" moving, living specimens. It can be applied to a range of cell-level biochemical processes, and we believe it could impact on the fields of drug action, cell physiology, pathology and disease as a whole.