Dynamics of site juxtaposition in supercoiled DNA
Dynamics of site juxtaposition in supercoiled DNA
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DOI：
10.1073/pnas.98.3.968
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发表时间：
2001-01-30
影响因子：
11.1
通讯作者：
Vologodskii, A
中科院分区：
文献类型：
--
作者：
Huang, J;Schlick, T;Vologodskii, A
Juxtaposition kinetics between specific sites in supercoiled DNA is investigated at close to physiological ionic conditions by Brownian dynamics simulations. At such conditions, supercoiled DNA is interwound, and the probability of spatial site juxtaposition is much higher than in relaxed DNA. We find, however, that supercoiling does not correspondingly increase the rate of juxtaposition at these physiological conditions. An explanation to this unexpected finding emerges on analysis of the juxtaposition dynamics. We note that although a particular site ii in supercoiled DNA is often in close proximity (juxtaposed) to another site ir, the change of it occurs very slowly and depends largely on internal slithering of opposite segments of the DNA superhelix. Such slithering results in long correlations between successive values of it; these correlations increase the average time of juxtaposition between two DNA sites. Random collisions between sites located on different superhelix branches-although increasing in importance with DNA size-contribute less substantially to site juxtaposition at high sa It than slithering for DNA up to 6 kb in length.
