Quantitation of rheumatoid factor by laser nephelometry
Quantitation of rheumatoid factor by laser nephelometry
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通过激光比浊法定量类风湿因子
DOI：
10.1007/bf00541265
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发表时间：
2004
影响因子：
4
通讯作者：
M. Ziff
中科院分区：
文献类型：
--
作者：
P. Roberts;R. Wernick;M. Ziff
激光散射比浊法是一种测量类风湿因子与热聚集的人免疫球蛋白相互作用引起的光散射程度的灵敏技术。在测定类风湿患者血清和滑液中类风湿因子(RF)水平时，用散射比浊法测定了IgM-RF和IgG-RF的总和。然而，在同等重量的基础上，IgM-RF产生的散射度至少是IgG-RF的17倍。低分子量IgM-RF在本实验中具有最小的光散射活性。散射比浊法测定的血清总RF水平与固相放射免疫法测定的μ-RF显著相关(r=0.97，n=30，P<；0.001)。结论：尽管类风湿因子的单克隆性或偶尔的多克隆性制备可能会给出不典型的结果，但比浊法RF试验是一种简单、快速的方法来测量各种生物体液中的总多克隆RF水平。
Laser nephelometry is a sensitive technique for measuring the degree of light scatter caused by the interaction of rheumatoid factors with heat aggregated human IgG. In measuring the rheumatoid factor (RF) level in rheumatoid serum and synovial fluid, it was found that nephelometry measured the combined amounts of IgM-RF and IgG-RF. However, on an equivalent weight basis, IgM-RF produced at least 17 times more scatter than IgG-RF. Low molecular weight IgM-RF had minimal light scattering activity in this assay. The total serum RF level, measured by nephelometry and expressed in terms of μg/ml of IgM-RF equivalents, correlated strongly with IgM-RF measured by solid phase radioimmunoassay (r=0.97,n=30,P<0.001) and an equation was derived to equate the two variables. It is concluded that the nephelometric RF test is a simple and rapid method to measure the total polyclonal RF level in various biological fluids, although monoclonal or an occasional polyclonal preparation of rheumatoid factor may give atypical results.