An in vivo 19F nuclear magnetic resonance study of isoflurane elimination from the rabbit brain.
An in vivo 19F nuclear magnetic resonance study of isoflurane elimination from the rabbit brain.
复制标题
兔脑中异氟烷消除的体内 19F 核磁共振研究。
DOI：
10.1097/00000542-198708000-00003
复制
发表时间：
1987
期刊：
影响因子：
8.8
通讯作者：
Litt,L
中科院分区：
文献类型：
--
作者：
Mills,P;Sessler,DI;Moseley,M;Chew,W;Pereira,B;James,TL;Litt,L
在2.0特斯拉下进行19 F核磁共振波谱分析，以评价麻醉90分钟后异氟烷从成年兔脑中的洗脱。本研究调和了其他人先前的体内NMR研究，观察到缓慢的麻醉剂洗脱，以及发现快速洗脱的侵入性非NMR研究，如麻醉剂摄取和消除的灌注限制模型所预测的。进行了两个NMR表面线圈实验：第一个实验中，将1 cm的表面线圈直接放置在暴露的硬脑膜上，以确保仅从大脑观察到冲洗;第二个实验中，将3 cm的线圈无创放置在完整的头皮上，以模拟先前的NMR实验。与之前的NMR实验一样，使用大线圈观察到异氟烷的缓慢洗脱。使用大线圈观察到的NMR信号不能仅归因于脑组织。大脑周围的脂肪对用3 cm线圈观察到的氟NMR谱有显著贡献，并且其贡献延长了表观洗脱时间。使用小线圈观察到异氟烷从兔脑中快速洗脱，其信号明确仅来自脑组织。所观察到的快速洗脱与先前从脑中的麻醉剂洗脱的侵入性生物化学测量一致。
19F nuclear magnetic resonance spectroscopy was performed at 2.0 Tesla to evaluate the washout of isoflurane from the adult rabbit brain after 90 min of anesthesia. This investigation reconciles previous in vivo NMR studies of others, which observed a slow anesthetic washout, with invasive non-NMR studies that found a rapid washout, as predicted by perfusion-limited models of anesthetic uptake and elimination. Two NMR surface coil experiments were performed: in the first, a 1-cm surface coil was placed directly over the exposed dura to be certain that the washout was observed only from the brain; in the second, a 3-cm coil was placed noninvasively over the intact scalp to emulate previous NMR experiments. As in previous NMR experiments, a slow washout of isoflurane was observed with the large coil. The NMR signal that is observed with the large coil cannot be attributed solely to brain tissue. Fat surrounding the brain contributes significantly to the fluorine NMR spectra that are observed with the 3-cm coil, and its contributions lengthen the apparent washout time. A rapid washout of isoflurane from the rabbit brain was observed with the small coil, whose signal unambiguously arises only from brain tissue. The observed rapid washout is consistent with previous invasive biochemical measurements of anesthetic washout from the brain.