Use of a monoclonal anti-estrogen receptor antibody in the immunohistochemical evaluation of human tumors.
Use of a monoclonal anti-estrogen receptor antibody in the immunohistochemical evaluation of human tumors.
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发表时间：
1986-08
期刊：
影响因子：
11.2
通讯作者：
K. Mccarty;E. Szabó;J. L. Flowers;E. Cox;G. Leight;Miller Ls;J. Konrath;J. Soper;Budwit Da-
中科院分区：
文献类型：
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作者：
K. Mccarty;E. Szabó;J. L. Flowers;E. Cox;G. Leight;Miller Ls;J. Konrath;J. Soper;Budwit Da-
用抗人雌激素受体蛋白（H222 Sp γ）单克隆抗体，经免疫过氧化物酶技术扩增，用于452例乳腺癌、100例子宫内膜癌和15例黑色素瘤雌激素受体的分析。免疫组织化学评价包括染色强度和分布（HSCORE）。通过葡聚糖包被的活性炭分析和蔗糖密度梯度分析来定量测定雌激素受体含量。在所有情况下，H222 Sp γ定位于靶细胞的细胞核中。乳腺和子宫内膜组织的HSCORE和生化检测之间存在半定量相关性。与生化测定相比，HSCORE的灵敏度和特异性范围分别为80%至95%和74%至94%。HSCORE与乳腺癌和子宫内膜癌的肿瘤分级相关。免疫组化评价显示，在黑色素瘤中没有特异性染色。这些数据表明，免疫组织化学受体定位提供的信息补充标准的生物化学测定在研究的组织。
A monoclonal antibody to human estrogen receptor protein (H222 Sp gamma), amplified via immunoperoxidase techniques, was used in the analysis of estrogen receptor in 452 breast carcinomas, 100 endometrial carcinomas, and 15 melanomas. Immunohistochemical evaluation incorporated both intensity and distribution of staining (HSCORE). Quantitative estrogen receptor content was determined by dextran-coated charcoal analysis and sucrose density gradient analysis. In all cases H222 Sp gamma localized in the nucleus of target cells. A semiquantitative correlation existed between HSCORE and biochemical assays for breast and endometrial tissues. The sensitivities and specificities for HSCORE as compared to the biochemical assays ranged from 80 to 95% and from 74 to 94%, respectively. HSCORE correlated with tumor grade for breast and endometrial carcinoma. Immunohistochemical evaluation showed no specific staining in melanomas. The data suggest that immunohistochemical receptor localization provides information complementary to standard biochemical assays in the tissues studied.