Neuritic guidance by polyornithine-attached materials of ganglionic origin.
Neuritic guidance by polyornithine-attached materials of ganglionic origin.
复制标题
神经节起源的聚鸟氨酸附着材料的神经炎引导。
DOI：
10.1016/0012-1606(81)90342-0
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发表时间：
1981
影响因子：
2.7
通讯作者：
Varon,SU
中科院分区：
文献类型：
--
作者：
Adler,R;Varon,SU
分离的睫状神经节神经元不能在培养中存活，除非提供适当来源的睫状神经营养因子(CNTF)。此外，该实验室和其他实验室之前的工作表明，多鸟氨酸(PON)底物本身不允许从CNTF支持的神经元发育成神经细胞。然而，如果色情基质上涂有来自心脏条件培养液的促进轴突因子(NPF)，就会发生神经细胞的形成。未分离的鸡胚睫状神经节移植到色情基质上，只有在CNTF支持下，在基质上涂上NPF，才能产生大量的神经突起。在没有外源性NPF的情况下，神经突起呈放射状生长，但范围有限。在距外植体约12 0μm处，轴突由放射状转变为环状或切向生长，从而形成一个几乎与外植体同心的神经环。时间推移研究表明，神经环是由几种行为的组合产生的，当神经突起到达径向到圆形的“过渡区”时，包括分支和顺时针或逆时针旋转。来自邻近生长锥的切向丝状足细胞融合形成的神经间桥也起到了作用。神经突起生长的整体行为表明，移植的睫状神经节能够产生具有促进轴突特性的材料，这些材料覆盖在神经节附近的色情底物上。用[~3H]氨基葡萄糖或[14C]亮氨酸预先标记神经节的放射自显影实验证明了这些物质的存在。神经节起源的色情附着物(PAM)的节周区域在移植后几个小时，神经元性突起之前就可以被识别出来。这一PAM区域大致对应于几小时后将被神经环占据的区域。如果两个神经节移位的距离大约等于神经节周围致密的PAM晕环通常覆盖的距离的两倍，则在神经炎突起开始之前，两个神经节的PAM区域之间就建立了连续性。这种重叠产生了一条“优先地形大道”，为相邻神经节之间的神经突起提供指导。我们认为，类似的神经学指导机制也可能在体内发生。
Dissociated ciliary ganglionic neurons do not survive in culture unless supplied with an appropriate source of ciliary neuronotrophic factors (CNTF). Moreover, previous work from this and other laboratories has shown that polyornithine (PORN) substrata per se do not allow neuritic development from such CNTF-supported neurons. Neuritic formation, however, would take place if the PORN substratum is coated with a neurite-promoting factor (NPF) from heart conditioned medium. Undissociated chick embryo ciliary ganglia explanted on PORN substrata will also produce a lavishradialneuritic outgrowth only if they are CNTF supported and the substratum is coated with NPF. In the absence of exogenous NPF neurites grow radially but only to a limited extent. At a distance of approximately 120 μm from the explant, neurites switch from a radial to a circular or tangential growth pattern, thus giving rise to a neuritic ring almost concentric with respect to the explant. Time-lapse studies show that the neuritic ring is generated through a combination of several behaviors that neurites display upon reaching the radial-to-circular “transition zone,” including branching and turning either clock- or counterclockwise. Contributions are also made by interneuritic bridges arising from fusion of tangential filopodia from adjacent growth cones. The overall behavior of neuritic outgrowth suggests that the explanted ciliary ganglia are able to produce materials endowed with neurite-promoting properties, which coat the PORN substratum in the immediate vicinity of the ganglia. Autoradiographic experiments using ganglia prelabeled with either [3H]glucosamine or [14C]leucine demonstrated the existence of these materials. A periganglionic territory of PORN-attached materials (PAMs) of ganglionic origin can be recognized a few hours after explantation, before the onset of neuritic emergence from the ganglion. This PAM territory grossly corresponds to the area that a few hours later will be occupied by the neuritic ring. If two ganglia are explanted at a distance approximately equal to twice the distance normally covered by a dense periganglionic PAM halo, a continuity between the PAM territories of both ganglia is established before the onset of neuritic outgrowth. This overlapping generates an “avenue of preferential terrain” that provides guidance to neurites between the neighboring ganglia. The possibility is considered that a similar mechanism of neuritic guidance could also occurin vivo.