High-sensitivity amino acid analysis: methodology for the determination of amino acid compositions with less than 100 picomoles of peptides.
High-sensitivity amino acid analysis: methodology for the determination of amino acid compositions with less than 100 picomoles of peptides.
复制标题
高灵敏度氨基酸分析：用少于 100 皮摩尔的肽测定氨基酸组成的方法。
DOI：
10.1016/0003-2697(82)90120-8
复制
发表时间：
1982
影响因子：
2.9
通讯作者：
Schroeder,R
中科院分区：
文献类型：
--
作者：
Böhlen,P;Schroeder,R
本文介绍了一种以邻苯二甲醛为检测剂的自动荧光氨基酸分析仪测定皮摩尔量多肽的氨基酸组成的高灵敏度方法。所有常见的氨基酸，包括胱氨酸、脯氨酸和色氨酸，都可以定量。高灵敏度主要是通过使用简单的程序来实现的，这些程序有效且可靠地降低了缓冲液和试剂中存在的或在样品处理过程中引入的干扰污染物的水平。用50 pmol或更少的肽获得准确和可再现的氨基酸组成。
Highly sensitive methodology is described for the determination of amino acid compositions of picomole quantities of peptides using an automated fluorescence amino acid analyzer with o-phthalaldehyde as the detection agent. All commonly occurring amino acids, including cystine, proline, and tryptophan, can be quantitated. High sensitivity is primarily achieved by using simple procedures which effectively and reliably reduce the level of interfering contamination present in buffers and reagents or introduced during sample handling. Accurate and reproducible amino acid compositions are obtained with 50 pmol or less peptide.