Succinate dehydrogenase activity of external and internal hyphae of a vesicular-arbuscular mycorrhizal fungus, Glomus mosseae (Nicol. & Gerd.) Gerdmann and Trappe, during mycorrhizal colonization of roots of leek (Allium porrum L.), as revealed by in situ histochemical staining
Succinate dehydrogenase activity of external and internal hyphae of a vesicular-arbuscular mycorrhizal fungus, Glomus mosseae (Nicol. & Gerd.) Gerdmann and Trappe, during mycorrhizal colonization of roots of leek (Allium porrum L.), as revealed by in situ histochemical staining
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囊泡丛枝菌根真菌 Glomus mosseae (Nicol.) 外部和内部菌丝的琥珀酸脱氢酶活性
DOI：
10.1007/bf00204059
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发表时间：
1993
期刊：
影响因子：
3.9
通讯作者：
C. Hepper
中科院分区：
文献类型：
--
作者：
M. Saito;D. P. Stribley;C. Hepper
研究了VA菌根真菌Glomus Mosseae(Nicol.&Gerd.)Gerdmann和Trappe，与大葱(Allium porum L.)用组织化学方法进行了原位观察。将大蒜幼苗移栽沙培，接种青枯菌孢子。苔藓恰好在茎的底部下方。移栽后每隔14天、25天、35天和60天，用硝基蓝氯化四氮唑溶液浸泡幼苗的生长液，以取代营养液。这使得菌根的外部和内部真菌结构的SDH活性部位被染色，而不会在物理上干扰共生系统。在收获的根和菌丝用酸性品红进行反染色后，可以清楚地区分该真菌的代谢活性区域和非活性区域。外生菌丝和病根的长度几乎呈指数增长，并且在60d内保持恒定比例(每厘米病根有1.4m菌丝)。外生菌丝长度与SDH活性的百分比几乎保持不变(80%)。在每个侵染长度的根中，SDH活性从不活跃的远端(老)菌丝到均匀活跃的近端(年轻)菌丝有一个等级。这些发现与共生菌的共生活性有关。
The succinate dehydrogenase (SDH) activity of hyphae of the vesicular-arbuscular (VA) mycorrhizal fungusGlomus mosseae(Nicol. & Gerd.) Gerdmann and Trappe, in symbiotic association with leek (Allium porrumL.) roots, was investigated by histochemical staining in situ. Leek seedlings were transplanted to sand culture and inoculated with spores ofG. mosseaeplaced just below the base of the stem. At intervals (14, 25, 35 and 60 days) after transplanting, the growth medium of seedlings was flooded with nitro blue tetrazolium chloride solution, thereby displacing the nutrient solution. This allowed sites of SDH activity of external and internal fungal structures of the mycorrhizas to be stained without physically disturbing the symbiotic system. After counterstaining harvested roots and mycelium with acid fuchsin, it was possible to differentiate clearly metabolically active and inactive regions of the fungus. The lengths of external hyphae and infected root both increased nearly exponentially, and were in constant proportion (1.4 m hyphae per cm of infected root) for up to 60 days. The percentage length of external hyphae with SDH activity remained almost constant (80%). In each infected length of root there was a gradation of SDH activity from inactive distal (older) hyphae to uniformly active proximal (younger) hyphae. These findings are discussed in relation to the symbiotic activity of the mycobiont.