Production of immunogenic West Nile virus-like particles using a herpes simplex virus 1 recombinant vector.
Production of immunogenic West Nile virus-like particles using a herpes simplex virus 1 recombinant vector.
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DOI：
10.1016/j.virol.2016.06.006
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发表时间：
2016-09
期刊：
影响因子：
3.7
通讯作者：
Knipe DM
中科院分区：
文献类型：
--
作者：
Taylor TJ;Diaz F;Colgrove RC;Bernard KA;DeLuca NA;Whelan SPJ;Knipe DM
西尼罗河病毒（WNV）是一种黄病毒，于1999年迅速席卷北美，流行率下降，然后在2012年死灰复燃。到目前为止，还没有疫苗可用于预防人群中的感染。单纯疱疹病毒（HSV）复制缺陷型疫苗载体诱导持久的免疫力，其特征在于强抗体和CD 8 + T细胞反应，即使在HSV免疫动物。在这项研究中，一个WNV蛋白表达盒进行了优化的病毒样颗粒（VLP）的生产在转染研究中，并将该盒重组到HSV-1 d106-WNV病毒载体，产生细胞外VLP，证实了免疫电子显微镜。用d106-WNV重组载体免疫小鼠引起特异性抗WNV IgG应答。这项研究强调了有效组装病毒样颗粒所需的黄病毒编码序列。这一信息将有助于产生针对其他黄病毒的额外疫苗载体，包括最近出现的寨卡病毒。
West Nile virus (WNV) is a flavivirus that swept rapidly across North America in 1999, declined in prevalence, and then resurged in 2012. To date, no vaccine is available to prevent infection in the human population. Herpes simplex virus (HSV) replication-defective vaccine vectors induce a durable immunity characterized by strong antibody and CD8+ T cell responses even in HSV-immune animals. In this study, a WNV protein expression cassette was optimized for virus-like particle (VLP) production in transfection studies, and the cassette was recombined into an HSV-1 d106-WNV virus vector, which produced extracellular VLPs, as confirmed by immunoelectron microscopy. Immunization of mice with the d106-WNV recombinant vector elicited a specific anti-WNV IgG response. This study highlights the flavivirus coding sequences needed for efficient assembly of virus-like particles. This information will facilitate generation of additional vaccine vectors against other flaviviruses including the recently emerged Zika virus.