Genome sequence of the streptomycin-producing microorganism Streptomyces griseus IFO 13350
Genome sequence of the streptomycin-producing microorganism Streptomyces griseus IFO 13350
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DOI：
10.1128/jb.00204-08
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发表时间：
2008-06-01
影响因子：
3.2
通讯作者：
Horinouchi, Sueharu
中科院分区：
文献类型：
--
作者：
Ohnishi, Yasuo;Ishikawa, Jun;Horinouchi, Sueharu
我们确定了灰链霉菌IFO 13350的全基因组序列，这是一种产生抗结核剂链霉素的土壤细菌，链霉素是60多年前发现的第一种氨基糖苷类抗生素。线性染色体由8,545,929个碱基对（bp）组成，平均G+C含量为72.2%，预测7138个开放阅读框，6个rRNA操作子（16S-23S-5S）和66个tRNA基因。它包含极长的末端倒置重复序列（TIRs），每个长度为132,910 bp。端粒的核苷酸序列和二级结构与其他链霉菌保守的典型端粒不同，由几个回文组成，环状序列为5‘-GGA-3’。根据这种差异，染色体具有一个保守的末端蛋白（Tpg）和一个端粒相关蛋白（Tap）的假基因，而一个新的Tpg和Tap蛋白对由tir编码。与两个亲缘种——colelicolstreptomyces A3(2)和avermitilstreptomyces的基因组比较，不仅明确了griseus基因组的特征，而且还明确了24种链霉菌特异性蛋白的存在。稻瘟病菌基因组包含34个基因簇或基因，用于已知或未知的次生代谢产物的生物合成。利用DNA芯片进行转录组分析表明，除了链霉素生物合成基因簇外，至少有4个这些基因簇被AdpA直接或间接激活，AdpA是灰稻谷菌a因子（γ -丁内酯信号分子）调控级联中的次级代谢和形态发生的中心转录激活因子。
We determined the complete genome sequence of Streptomyces griseus IFO 13350, a soil bacterium producing an antituberculosis agent, streptomycin, which is the first aminoglycoside antibiotic, discovered more than 60 years ago. The linear chromosome consists of 8,545,929 base pairs (bp), with an average G+C content of 72.2%, predicting 7,138 open reading frames, six rRNA operons (16S-23S-5S), and 66 tRNA genes. It contains extremely long terminal inverted repeats (TIRs) of 132,910 bp each. The telomere's nucleotide sequence and secondary structure, consisting of several palindromes with a loop sequence of 5'-GGA-3', are different from those of typical telomeres conserved among other Streptomyces species. In accordance with the difference, the chromosome has pseudogenes for a conserved terminal protein (Tpg) and a telomere-associated protein (Tap), and a novel pair of Tpg and Tap proteins is instead encoded by the TIRs. Comparisons with the genomes of two related species, Streptomyces coelicolor A3(2) and Streptomyces avermitilis, clarified not only the characteristics of the S. griseus genome but also the existence of 24 Streptomyces-specific proteins. The S. griseus genome contains 34 gene clusters or genes for the biosynthesis of known or unknown secondary metabolites. Transcriptome analysis using a DNA microarray showed that at least four of these clusters, in addition to the streptomycin biosynthesis gene cluster, were activated directly or indirectly by AdpA, which is a central transcriptional activator for secondary metabolism and morphogenesis in the A-factor (a gamma-butyrolactone signaling molecule) regulatory cascade in S. griseus.