SLIRP Interacts with Helicases to Facilitate 2′-O-Methylation of rRNA and to Promote Translation
SLIRP Interacts with Helicases to Facilitate 2′-O-Methylation of rRNA and to Promote Translation
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DOI：
10.1021/jacs.9b04424
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发表时间：
2019-07-17
影响因子：
15
通讯作者：
Wang, Yinsheng
中科院分区：
文献类型：
--
作者：
Li, Lin;Miao, Weili;Wang, Yinsheng
SRA茎环相互作用RNA结合蛋白（SLIRP）是一种多功能蛋白，可以与RNA中的茎环结构和G四链体DNA相互作用。通过定量蛋白质组学实验，我们发现SLIRP与大多数人解旋酶蛋白质组相互作用。我们还发现这些相互作用促进rRNA中许多核苷的2 '-O-甲基化并促进蛋白质翻译。因此，我们发现了SLIRP蛋白的一种新功能，并对其作为RNA伴侣的功能和rRNA的2 '-O-甲基化的调节提供了新的机制见解。
SRA stem-loop-interacting RNA-binding protein (SLIRP) is a versatile protein that can interact with the stem-loop structure in RNA and with G quadruplex DNA. By using a quantitative proteomic experiment, we found that SLIRP interacts with the majority of the human helicase proteome. We also found that these interactions facilitate 2'-O-methylation of a number of nucleosides in rRNA and promote protein translation. Hence, we uncovered a novel function of SLIRP protein and offered novel mechanistic insights into its function as a RNA chaperone and into the regulation of 2'-O-methylation of rRNA.