Evolutionary conservation of a unique amino acid sequence in human DICER protein essential for binding to Argonaute family proteins
Evolutionary conservation of a unique amino acid sequence in human DICER protein essential for binding to Argonaute family proteins
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DOI：
10.1016/j.gene.2007.04.001
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发表时间：
2007-07-15
期刊：
影响因子：
3.5
通讯作者：
Shimizu, Nobuyoshi
中科院分区：
文献类型：
--
作者：
Sasaki, Takashi;Shimizu, Nobuyoshi
Argonaute 家族和 DICER 蛋白是参与各个物种 RNA 介导的基因沉默机制的主要关键蛋白。在此机制中，信使 RNA (mRNA) 的裂解或 mRNA 翻译的抑制是通过 DICER 独特加工的小 RNA 进行的。之前，我们证明了人类 Argonaute 家族蛋白与 DICER 结合。在这项研究中，我们在人 DICER 蛋白的 RIBOc-A 结构域中鉴定了一个由 127 个氨基酸组成的独特氨基酸序列，作为 Argonaute 蛋白的“结合位点”。比较基因组学分析表明，这种独特的氨基酸序列在脊椎动物中高度保守，但在非脊椎动物物种中没有发现。脊椎动物和非脊椎动物物种之间 DICER 蛋白 RIBOc-A 结构域的显着差异可能有助于探索 RNA 介导的基因沉默机制的功能复杂性。 (C) 2007 Elsevier B.V. 保留所有权利。
The Argonaute family and DICER proteins are major key proteins involved in the RNA-mediated gene silencing mechanism of various species. In this mechanism, cleavage of messenger RNAs (mRNA) or suppression of mRNA translation takes place via small RNAs that are uniquely processed by DICER. Previously, we demonstrated that human Argonaute family proteins bind to DICER. In this study, we identified a unique amino acid sequence of 127 amino acids in the RIBOc-A domain of human DICER protein as a "binding site" to Argonaute proteins. Comparative genomics analysis revealed that this unique amino acid sequence is highly conserved in the vertebrates, but not found in the nonvertebrate species. Significant difference in the RIBOc-A domain of DICER protein between vertebrate and non-vertebrate species may help exploring the functional complexity in the RNA-mediated gene silencing mechanism. (C) 2007 Elsevier B.V. All rights reserved.