Separation of isomeric peptides using electrospray ionization/high-resolution ion mobility spectrometry.
Separation of isomeric peptides using electrospray ionization/high-resolution ion mobility spectrometry.
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DOI：
10.1021/ac990601c
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发表时间：
2000-01
影响因子：
7.4
通讯作者：
Ching Wu;William F. Siems;Jörg Klasmeier;H. H. Hill-H.
中科院分区：
文献类型：
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作者：
Ching Wu;William F. Siems;Jörg Klasmeier;H. H. Hill-H.
本文首次报道了大气压下电喷雾离子迁移谱（ESI/IMS）基线分离同分异构大分子的实例。使用氮气作为漂移气体研究了IMS中许多不同异构体肽的行为。IMS耦合到四极杆质谱仪，用于识别和选择性检测的电喷雾离子。迁移率数据被用来确定它们的平均碰撞截面。异构肽的气相离子被发现有不同的碰撞截面。在所有情况下，双电荷离子表现出显着（8-20%）较大的碰撞截面比各自的单电荷物种。异构体肽的混合物的分析清楚地表明IMS分离气相肽离子的能力，由于它们的构象结构的微小差异，这不能通过质谱法确定。两个双电荷反向测序五肽的实际分辨率为80。基线分离提供的离子不同，只有2.5%，在其测得的碰撞截面;部分分离显示异构体离子表现出的差异小至1.1%。
In this paper, the first examples of baseline separation of isomeric macromolecules by electrospray ionization/ion mobility spectrometry (ESI/IMS) at atmospheric pressure are presented. The behavior of a number of different isomeric peptides in the IMS was investigated using nitrogen as a drift gas. The IMS was coupled to a quadrupole mass spectrometer, which was used for identification and selective detection of the electrosprayed ions. The mobility data were used to determine their average collision cross sections. The gas-phase ions of isomeric peptides were found to have different collision cross sections. In all cases, doubly charged ions exhibited significantly (8-20%) larger collision cross sections than the respective singly charged species. The analysis of mixtures of the isomeric peptides clearly demonstrated the capability of IMS to separate gas-phase peptide ions due to small differences in their conformational structures, which cannot be determined by mass spectrometry. An actual resolving power of 80 was achieved for two doubly charged reversed sequenced pentapeptides. Baseline separation was provided for ions differing by only 2.5% in their measured collision cross sections; partial separation was shown for isomeric ions exhibiting differences as small as 1.1%.