SOX11 expression is highly specific for mantle cell lymphoma and identifies the cyclin D1-negative subtype
SOX11 expression is highly specific for mantle cell lymphoma and identifies the cyclin D1-negative subtype
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DOI：
10.3324/haematol.2009.010264
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发表时间：
2009-11-01
期刊：
影响因子：
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通讯作者：
Campo, Elias
中科院分区：
文献类型：
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作者：
Mozos, Ana;Royo, Cristina;Campo, Elias
背景细胞周期蛋白 D1 阴性套细胞淋巴瘤很难与其他小 B 细胞淋巴瘤区分开。这种淋巴瘤患者的临床和病理特征尚未明确。在传统套细胞淋巴瘤中观察到转录因子 SOX11 过度表达。本研究的目的是确定该基因是否在细胞周期蛋白 D1 阴性套细胞淋巴瘤中表达，以及其检测是否可用于识别这些肿瘤。设计和方法重新检查了 238 个成熟 B 细胞肿瘤的微阵列数据库。采用免疫组化方法检测 12 例细胞周期蛋白 D1 阴性套细胞淋巴瘤、54 例传统套细胞淋巴瘤和 209 例其他淋巴肿瘤中 SOX11 蛋白的表达。结果 SOX11 mRNA 在传统套细胞淋巴瘤和细胞周期蛋白 D1 阴性套细胞淋巴瘤以及 33% 的伯基特淋巴瘤病例中高表达，但在任何其他成熟淋巴肿瘤中均不表达。 SOX11 核蛋白在 50 例 (93%) 传统套细胞淋巴瘤、12 例细胞周期蛋白 D1 阴性的套细胞淋巴瘤、6 例淋巴母细胞淋巴瘤、8 例伯基特淋巴瘤中的 2 例以及 3 例 T 幼淋巴细胞白血病中的 2 例中检测到，但在其余淋巴肿瘤中呈阴性。细胞周期蛋白 D1 阴性套细胞淋巴瘤中细胞周期蛋白 D2 和 D3 mRNA 水平显着高于常规套细胞淋巴瘤，但蛋白表达没有差异。 SOX11表达鉴定的cyclin D1阴性套细胞淋巴瘤患者的临床病理特征和转归与传统套细胞淋巴瘤患者相似。结论SOX11 mRNA和核蛋白表达是cyclin D1阳性和阴性套细胞淋巴瘤的高度特异性标志物。
BackgroundCyclin D1-negative mantle cell lymphoma is difficult to distinguish from other small B-cell lymphomas. The clinical and pathological characteristics of patients with this form of lymphoma have not been well defined. Overexpression of the transcription factor SOX11 has been observed in conventional mantle cell lymphoma. The aim of this study was to determine whether this gene is expressed in cyclin D1-negative mantle cell lymphoma and whether its detection may be useful to identify these tumors.Design and MethodsThe microarray database of 238 mature B-cell neoplasms was re-examined. SOX11 protein expression was investigated immunohistochemically in 12 cases of cyclin D1-negative mantle cell lymphoma, 54 cases of conventional mantle cell lymphoma, and 209 additional lymphoid neoplasms.ResultsSOX11 mRNA was highly expressed in conventional and cyclin D1-negative mantle cell lymphoma and in 33% of the cases of Burkitt's lymphoma but not in any other mature lymphoid neoplasm. SOX11 nuclear protein was detected in 50 cases (93%) of conventional mantle cell lymphoma and also in the 12 cyclin D1-negative cases of mantle cell lymphoma, the six cases of lymphoblastic lymphomas, in two of eight cases of Burkitt's lymphoma, and in two of three T-prolymphocytic leukemias but was negative in the remaining lymphoid neoplasms. Cyclin D2 and D3 mRNA levels were significantly higher in cyclin D1-negative mantle cell lymphoma than in conventional mantle cell lymphoma but the protein expression was not discriminative. The clinico-pathological features and outcomes of the patients with cyclin D1-negative mantle cell lymphoma identified by SOX11 expression were similar to those of patients with conventional mantle cell lymphoma.ConclusionsSOX11 mRNA and nuclear protein expression is a highly specific marker for both cyclin D1-positive and negative mantle cell lymphoma.