Differential gene expression profiling between wild-type and ALAS2-null erythroblasts: Identification of novel heme-regulated genes
Differential gene expression profiling between wild-type and ALAS2-null erythroblasts: Identification of novel heme-regulated genes
复制标题
DOI：
10.1016/j.bbrc.2005.11.163
复制
发表时间：
2006-02-03
影响因子：
3.1
通讯作者：
Sasaki, T
中科院分区：
文献类型：
--
作者：
Fujiwara, T;Harigae, H;Sasaki, T
为了鉴定红系特异性血红素调节基因，我们对野生型和血红素缺陷型成红细胞之间进行了差异表达分析，这些成红细胞分别由野生型和红系特异性6-氨基特维酸合酶无效的小鼠ES细胞制备。在cDNA阵列上的8737个克隆中，首先根据其在野生型成红细胞中的高表达谱选择了40个cDNA克隆，包括34个未知的EST，并进一步评估了它们的红系谱系特异性、体内造血组织中的表达以及血红素依赖性表达，分别产生了11、4和4个基因。由于所采用的选择策略，最后 4 个被认为是新鉴定的红细胞特异性血红素调节基因。这 4 个基因分别是解偶联蛋白 2、核仁纺锤体相关蛋白、细胞核酸结合蛋白和一种新型乙酰转移酶样蛋白。因此，这些发现表明血红素可能调节多种迄今为止未被识别的基因，对这些基因的进一步分析可能会阐明它们在红系细胞分化中的作用。 (c) 2005 Elsevier Inc. 保留所有权利。
To identify erythroid-specific heme-regulated genes, we performed differential expression analysis between wild-type and heme-deficient erythroblasts, which had been prepared from wild-type and erythroid-specific 6-aminotevulinate synthase-null mouse ES cells, respectively. Among 8737 clones on cDNA array, 40 cDNA clones, including 34 unknown ESTs, were first selected by their high expression profiles in wild-type erythroblasts, and evaluated further for their erythroid-lineage specificity, expression in hematopoietic tissues in vivo, and heme-dependent expression, which yielded 11, 4, and 4 genes, respectively. Because of the selection strategy employed, the final 4 were considered as the newly identified erythroid-specific heme-regulated genes. These 4 genes were uncoupling protein 2, nucleolar spindle-associated protein, cellular nucleic acid-binding protein, and a novel acetyltransferase-like protein. These findings thus suggest that heme may regulate a wide variety of hitherto unrecognized genes, and further analysis of these genes may clarify their role in erythroid cell differentiation. (c) 2005 Elsevier Inc. All rights reserved.