The transcriptional landscape of the cultured murine middle ear epithelium in vitro.
The transcriptional landscape of the cultured murine middle ear epithelium in vitro.
复制标题
体外培养小鼠中耳上皮细胞的转录图谱。
DOI：
10.1242/bio.056564
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发表时间：
2021-04-15
期刊：
影响因子：
2.4
通讯作者：
Bingle CD
中科院分区：
文献类型：
--
作者：
Mulay A;Chowdhury MMK;James CT;Bingle L;Bingle CD
中耳炎 (OM) 是最常见的儿科疾病，发病率很高。尽管复杂的病理生理学阻碍了对潜在疾病机制的理解，但很明显上皮异常是该疾病的基础。 OM 中上皮重塑的机制仍不清楚。我们最近描述了一种新型的小鼠中耳上皮细胞（mMEEC）体外模型，该模型经历粘液纤毛分化为中耳腔中可见的各种上皮细胞群。我们现在描述 mMEEC 在分化时的全基因组基因表达谱。我们比较了原始（未培养）中耳细胞、未分化细胞的汇合培养物和在气液界面（ALI）中分化 7 天的细胞的基因表达谱。三组细胞之间差异表达超过 5000 个基因。原始细胞和 ALI 培养第 0 天之间大约有 4000 个基因存在差异表达。原始细胞群被证明含有多种细胞类型的混合物，包括在培养中丢失的污染性炎症细胞。在 ALI 诱导的分化过程中，大约 500 个基因上调。其中包括一些分泌基因和一些酶，但大多数与纤毛发生过程有关。数据表明，分化的小鼠中耳上皮的体外模型表现出与中耳内看到的粘膜纤毛上皮一致的转录谱。了解这种上皮细胞的转录景观将为理解 OM 小鼠模型中观察到的表型变化提供基础。摘要：本文介绍了小鼠中耳上皮细胞在分化为代表天然中耳上皮的粘膜纤毛表型时的全基因组转录分析。
Otitis media (OM) is the most common paediatric disease and leads to significant morbidity. Although understanding of underlying disease mechanisms is hampered by complex pathophysiology, it is clear that epithelial abnormalities underpin the disease. The mechanisms underpinning epithelial remodelling in OM remain unclear. We recently described a novel in vitro model of mouse middle ear epithelial cells (mMEECs) that undergoes mucociliary differentiation into the varied epithelial cell populations seen in the middle ear cavity. We now describe genome wide gene expression profiles of mMEECs as they undergo differentiation. We compared the gene expression profiles of original (uncultured) middle ear cells, confluent cultures of undifferentiated cells and cells that had been differentiated for 7 days at an air liquid interface (ALI). >5000 genes were differentially expressed among the three groups of cells. Approximately 4000 genes were differentially expressed between the original cells and day 0 of ALI culture. The original cell population was shown to contain a mix of cell types, including contaminating inflammatory cells that were lost on culture. Approximately 500 genes were upregulated during ALI induced differentiation. These included some secretory genes and some enzymes but most were associated with the process of ciliogenesis. The data suggest that the in vitro model of differentiated murine middle ear epithelium exhibits a transcriptional profile consistent with the mucociliary epithelium seen within the middle ear. Knowledge of the transcriptional landscape of this epithelium will provide a basis for understanding the phenotypic changes seen in murine models of OM. Summary: This paper presents a genome wide transcriptional analysis of murine middle ear epithelial cells as they undergo differentiation to a mucociliary phenotype representative of the native middle ear epithelium.
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影响因子：
4.5
作者：
Crompton M;Purnell T;Tyrer HE;Parker A;Ball G;Hardisty-Hughes RE;Gale R;Williams D;Dean CH;Simon MM;Mallon AM;Wells S;Bhutta MF;Burton MJ;Tateossian H;Brown SDM
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影响因子：
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作者：
Azar A;Piccinelli C;Brown H;Headon D;Cheeseman M
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DOI：
10.1073/pnas.0813216106
发表时间：
2009-09-08
影响因子：
11.1
作者：
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通讯作者：
Gustincich, Stefano
影响因子：
4.6
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Burton MJ
影响因子：
4.6
作者：
Mulay A;Hood DW;Williams D;Russell C;Brown SDM;Bingle L;Cheeseman M;Bingle CD
通讯作者：
Bingle CD