Pulsed field gel electrophoresis analysis conditions for molecular epidemiological identification of vertical Group B Streptococcus infection.

Pulsed field gel electrophoresis analysis conditions for molecular epidemiological identification of vertical Group B Streptococcus infection.
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垂直B族链球菌感染分子流行病学鉴定的脉冲场凝胶电泳分析条件。

DOI:
10.11150/kansenshogakuzasshi1970.79.561
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发表时间:
2005
期刊:
Kansenshogaku zasshi. The Journal of the Japanese Association for Infectious Diseases
影响因子:
--
通讯作者:
M. Mizokami
M. Mizokami
中科院分区:
--
文献类型:
--
作者:
H. Wakimoto;H. Yano;S. Baba;K. Okuzumi;Noriko Okamoto;Y. Wakimoto;S. Miyakawa;M. Mizokami

文献摘要

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B群链球菌(GBS)是一种病原体,有通过产道垂直感染的风险,但也有报道称托儿所发生了水平感染。脉冲场凝胶电泳法(PFGE)能够对感染途径进行分子流行病学调查,但存在耗时过长的缺点。银杏叶提取物的PFGE分析条件差异很大。我们通过分子流行病学优化了确定垂直GBS感染的变量。
Group B Streptococcus(GBS)is a pathogen with a risk of vertical infection through the birth canal, but reports have also been made of horizontal infection in the nursery. Pulsed field gel electrophoresis (PFGE)enables molecular epidemiologic investigation of the infection route, but has the drawback of taking too much time. Conditions for PFGE analysis for GBS vary greatly. We optimized variables for identifying vertical GBS infection by molecular epidemiology.