Development of Neutral Liposome Plasmid DNA Complexes for Gene Transfer: Non-Viral Gene Delivery Systems
Development of Neutral Liposome Plasmid DNA Complexes for Gene Transfer: Non-Viral Gene Delivery Systems
复制标题
用于基因转移的中性脂质体质粒 DNA 复合物的开发：非病毒基因传递系统
DOI：
10.1021/bk-2004-0879.ch005
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发表时间：
2004
期刊：
影响因子：
--
通讯作者：
S. Sullivan
中科院分区：
文献类型：
--
作者：
A. Iyengar;S. Sullivan
基于质粒的基因递送系统依赖于使用阳离子两亲物将基因递送至细胞。下一章描述了质粒DNA中性脂质体包装系统的开发，该系统具有高捕获效率（70%），脂质体直径为180 nm，与单独的质粒或与阳离子两亲物一起配制时相比，从血流中的清除速率较慢，并且可以容纳靶向配体的掺入，同时保留粒径，并且质粒捕获效率不降低。
Plasmid based gene delivery systems have relied on the use of cationic amphiphiles to deliver genes to cells. The following chapter describes the development of a neutral liposome packaging system for plasmid DNA that yields high trapping efficiency (70%), liposomes diameter of 180 nm, has slow clearance rates from the blood stream compared to plasmid alone or when formulated with cationic amphiphiles, and can accommodate incorporation of targeting ligands with retention of particle size and no decrease in plasmid trapping efficiency.