Hepatic stellate cells increase in Toxoplasma gondii infection in mice.
Hepatic stellate cells increase in Toxoplasma gondii infection in mice.
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DOI：
10.1186/1756-3305-6-135
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发表时间：
2013-05-04
影响因子：
3.2
通讯作者：
Kul O
中科院分区：
文献类型：
--
作者：
Atmaca HT;Gazyagcı AN;Canpolat S;Kul O
弓形虫是一种普遍存在的原生动物寄生虫，可以感染人类和动物。弓形虫病的严重程度根据个体、寄生虫株和宿主物种的免疫状态而变化。在哺乳动物物种中，已经观察到急性弓形虫病的严重病变在内脏器官如肝、肺和脾中形成。一些流行病学研究报道了T。肝硬化合并弓形虫感染。在15只30天大的正常瑞士白化病小鼠中诱导急性感染。小鼠腹腔接种5000 T.弓形虫RH株速殖子。在接种后2、4和6天，将小鼠以5只为一组处死。另一组5只小鼠用作对照。抗胶质细胞酸性蛋白（GFAP）和抗T.用弓形虫抗体比较GFAP免疫反应阳性细胞和抗T.在T.感染组和健康对照组。与T.弓形虫抗原免疫组化（P < 0.05）。T.随着HSC数量的增加，弓形虫免疫染色明显增强。肝星状细胞的数量与T. gondii抗原，这可能代表了HSC在肝脏病理学和T.刚地氏菌相关肝炎
Toxoplasma gondii is a ubiquitous protozoan parasite that can infect humans and animals. The severity of toxoplasmosis varies according to the immune status of the individual, parasite strain, and host species. In mammalian species, it has been observed that severe lesions of acute toxoplasmosis form in visceral organs such as the liver, lung, and spleen. Some epidemiological studies have reported an association of T. gondii infection with liver cirrhosis. Acute infection was induced in fifteen 30-day-old normal Swiss albino mice. The mice were infected by intraperitoneal inoculation of 5000 T. gondii RH strain tachyzoites. The mice were sacrificed in groups of 5 at 2, 4, and 6 days after inoculation. Another group of 5 mice were used as the controls. Anti-glial fibrillary acidic protein (GFAP) and anti-T. gondii antibodies were used to compare GFAP-immunoreactive cells and anti-T. gondii–immunopositive areas in the liver between the T. gondii-infected groups and the healthy controls, respectively. There was a significant correlation between the numbers of GFAP-positive hepatic stellate cells (HSCs) when they were compared with T. gondii antigen immunostaining (p < 0.05). The amount of T. gondii immunostaining increased significantly with the increase in the number of HSCs. There is a significant relationship between the number of HSCs and T. gondii antigens, which may represent an active role of HSCs in liver pathology and the pathobiology of T. gondii-related hepatitis.