Cloning and sequencing of a putative acetylcholinesterase cDNA from Boophilus microplus (Acari: Ixodidae)
Cloning and sequencing of a putative acetylcholinesterase cDNA from Boophilus microplus (Acari: Ixodidae)
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DOI：
10.1093/jmedent/36.6.764
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发表时间：
1999-11-01
影响因子：
2.1
通讯作者：
Wagner, GG
中科院分区：
文献类型：
--
作者：
Hernandez, R;He, HQ;Wagner, GG
使用的策略的基础上来自其他物种的乙酰胆碱酯酶（AChE）的简并引物，我们克隆和测序一个假定的，AChE cDNA从南方牛蜱。微小牛蜱（Canestrini）。该序列与GenBank中发表的乙醚类AChE序列具有高度的同源性。开放阅读框为1，689 bp，对应于563个氨基酸的推导序列，具有AChE家族共有的保守区域和特征，这是其催化活性所必需的。有机磷杀螨剂（OP）抗性和敏感品系的推定cDNA序列没有差异。结果表明，该推定的AChE基因不参与抗性OP化合物作为一个突变的基因在耐药菌株的研究。然而，在用限制性核酸酶消化来自不同菌株的基因组DNA后，用来自该cDNA的探针在DNA片段中检测到差异。这表明该基因在B中具有多态性。微加。
Using a strategy based on degenerate primers derived from acetylcholinesterase (AChE) from other species, we cloned and sequenced a putative, AChE cDNA from the southern cattle tick. Boophilus microplus (Canestrini). The sequence has a high degree of homology to sequences of AChE from ether species reported in the GenBank. The open reading frame of 1,689 bp, corresponding to a deduced sequence of 563 amino acids, has conserved regions and features shared by the AChE family, necessary for its catalytic activity. No differences were found in the putative cDNA sequences from organophosphorus acaricide (OP) resistant and susceptible strains. The results suggest that this putative AChE gene is not involved in resistance to OP compounds as a mutated gene in the resistant strain studied. However, differences were detected, with a probe derived from this cDNA, in DNA fragments after digestion of genomic DNA from different strains with restriction nucleases. This indicates polymorphism in this gene in B. microplus.