A TAL effector repeat architecture for frameshift binding
A TAL effector repeat architecture for frameshift binding
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DOI：
10.1038/ncomms4447
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发表时间：
2014-03-01
影响因子：
16.6
通讯作者：
Boch, Jens
中科院分区：
文献类型：
--
作者：
Richter, Annekatrin;Streubel, Jana;Boch, Jens
转录激活因子样效应子（TALE）是黄单胞菌属重要的毒力因子，通过一个独特的34个氨基酸的串联重复结构域结合DNA，诱导植物基因的表达。到目前为止，TALE重复被描述为作为连续阵列结合到连续DNA序列，其中每个重复独立地识别单个DNA碱基。这种模块化的蛋白质结构使得能够为生物技术应用设计任何所需的DNA结合特异性。在这里，我们报告说，天然TALE重复不寻常的氨基酸序列长度打破了严格的一个重复一个碱基对结合模式，并介绍了当地的灵活性TALE DNA结合。这种灵活性允许TALE和TALE核酸酶识别具有单核苷酸缺失的靶序列变体。这种灵活性还允许TALE在等位基因启动子处激活转录，否则这些启动子赋予对宿主植物的抗性。
Transcription activator-like effectors (TALEs) are important Xanthomonas virulence factors that bind DNA via a unique tandem 34-amino-acid repeat domain to induce expression of plant genes. So far, TALE repeats are described to bind as a consecutive array to a consecutive DNA sequence, in which each repeat independently recognizes a single DNA base. This modular protein architecture enables the design of any desired DNA-binding specificity for biotechnology applications. Here we report that natural TALE repeats of unusual aminoacid sequence length break the strict one repeat-to-one base pair binding mode and introduce a local flexibility to TALE-DNA binding. This flexibility allows TALEs and TALE nucleases to recognize target sequence variants with single nucleotide deletions. The flexibility also allows TALEs to activate transcription at allelic promoters that otherwise confer resistance to the host plant.